LOCUS       sequence-151426-        9656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9656)
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B...
FEATURES             Location/Qualifiers
     source          1..9656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1060..1185
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1682..1915
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2100..2144
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2442..2559
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2618..3328)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2896..2915)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3165..3183
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3325..3334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3347..3724)
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     misc_feature    4914..5005
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(4946..4965)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    5019..5067
                     /note="synthetic polyadenylation signal"
     enhancer        5163..5466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5467..5670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5709..5718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5715..5717,5718..5720,5721..6434)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5715..5717,5718..5720,5721..6431)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5760..5781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6021..6040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6368..6389
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6454..6487)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6543..7131
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6596..6616)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7014..7025)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7134..7150)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7135..7151)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7660..7840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7902..8490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7982..8001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8661..9452,9453..9521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9284..9303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9522..9626)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     1081 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     1141 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     1201 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     1261 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     1321 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     1381 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     1441 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     1501 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
     1561 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1621 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1681 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1741 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1801 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1861 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1921 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1981 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     2041 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     2101 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     2161 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     2221 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2281 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2341 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
     2401 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     2461 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2521 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     2581 agagatccag tttggttagt accgggcccg ctctagatta cttgtacagc tcgtccatgc
     2641 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
     2701 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
     2761 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
     2821 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
     2881 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
     2941 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
     3001 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
     3061 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
     3121 actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct
     3181 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
     3241 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
     3301 tgttatcctc ctcgcccttg ctcaccatgg tggcgaccgg tggatcctta gagctagtgt
     3361 acttggtaac tgccttagtg ccctcggaca cagcatgctt agccagctcc ccaggcagca
     3421 gcaggcgcac agccgtctga atctccctgg aggtgatggt cgagcgctta ttgtagtgag
     3481 ccaggcgaga agcctcgccc gcgatgcgct cgaagatgtc gttgacgaag gagttcatga
     3541 tccccatggc cttggatgag atgccggtgt cggggtggac ctgcttcaga accttgtaca
     3601 catagataga atagctctcc ttgcggctgc gcttacgctt cttaccatcc ttcttctgcg
     3661 ccttagtgat agccttctta gaaccctttt taggggctgg agcagactta gagggttcag
     3721 gcatggtggc ggtaccaagc ttggaagaga gtgaggacga acgcgccccc accccctttt
     3781 atagcccaga tctcgagccc gggctagcac gcgtgtctgc taggtctgca ccgagttgct
     3841 gtgtgggact tgtctgttct gcaatacctt ttatatccac tgcctgagcc cctaatgtaa
     3901 gcaggagggg caggagatgc tgccaggact aattgggggt catgtgcgca ataggtcaaa
     3961 cccaaaggat gctgtggttg gtgactgcag ctgccggggc gaaggcctgg aaggggaggg
     4021 taggacacaa tcagaggctg ccgggtcctg acatggcccc agacctcttc ccggaggggc
     4081 tgctccagca cccctactgt gtgccatctc tctcagcctc agctggagca ctggctggcc
     4141 ctagagacga gctccttaga gcatagccta gagagggtac agagatctaa aatcggtttg
     4201 ggacaaactg gctccaactc gcatagcctg tgattttcag tgtctgccgt gagagcagga
     4261 gccaaacccc aggaaggagc ggtcagtaat gtcctcattc acccagtgca gggcagctcc
     4321 gctcatccag tggggagaag ggtggggaag tgtcctgctt agttttctgt caacttgaaa
     4381 gaagaaagcc tcaattgaga aaatgcctcc ataagatccg gttgtagggc atttcttatt
     4441 tagtggtttt gatgggggag agcccaaccc attgtgagtg gtgccacccc tgggccttgg
     4501 tcttgagttc tacaagacag caggctgagc aagccatgat gaacaagcca gtaagctgca
     4561 cttctccatg gcttcgagct tctgcctcaa ggttctagcc ctgcttgtgt cagagacacc
     4621 ccacatgaag ctcaagatgc ctgggacccc tgatgacaag gaagaaaaac ctgccgggtt
     4681 accgtatttc agagcaatgg ccatggtctc ttggggccca gtatgagtac tgagaggccc
     4741 caaggcccgc ttctttggtc caaacacctt gggatgtcct ttttgcaatt cttagtactt
     4801 gtcactcttt ttatttactg ttccttttct ttcttccttc cttgattaaa ctggtatttg
     4861 gtgacctggc atgatctagt aggagcccac ttgatatcca ggtacctatc gatagagaaa
     4921 tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt tttgtttcgt
     4981 tttgttttga tggagagcgt atgttagtac tatcgattca cacaaaaaac caacacacag
     5041 atgtaatgaa aataaagata ttttattgcg gccgcgtgga taaccgtatt accgccatgc
     5101 attagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5161 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5221 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5281 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     5341 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     5401 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     5461 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     5521 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     5581 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     5641 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
     5701 taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     5761 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     5821 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     5881 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     5941 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6001 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6061 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     6121 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     6181 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     6241 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6301 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     6361 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     6421 agctgtacaa gtaggaattc gtcgagggac ctaataactt cgtatagcat acattatacg
     6481 aagttataca tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg
     6541 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6601 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6661 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6721 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6781 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6841 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6901 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6961 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7021 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7081 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     7141 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7201 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7261 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7321 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7381 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     7441 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     7501 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     7561 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     7621 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     7681 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7741 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7801 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
     7861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8341 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8461 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8701 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8761 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8821 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8881 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8941 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9001 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9061 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9121 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9181 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9241 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9301 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9361 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9421 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9481 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9541 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9601 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgt
//
