LOCUS       sequence-151431-       10708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Golomb E, Ma X, Jana SS, Preston YA, Kawamoto S, Shoham NG, Goldin 
            E, Conti MA, Sellers JR, Adelstein RS
  TITLE     Identification and characterization of nonmuscle myosin II-C, a new 
            member of the myosin II family.
  JOURNAL   J Biol Chem. 2004 Jan 23. 279(4):2800-8.
  PUBMED    14594953
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Jan 23. 279(4):2800-8."
COMMENT     Sequence Label: pEGFP-NMHC II-C
FEATURES             Location/Qualifiers
     source          1..10708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      664..673
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6655..6664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6661..6663,6664..6666,6667..7380)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6661..6663,6664..6666,6667..7377)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6706..6727)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6967..6986)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7314..7335
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7503..7624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7540..7559)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7594..7613
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7631..8086)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7768..7789)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7980..7999
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8113..8217
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8219..8576
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8219..8410
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8242..8262)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8427..8562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8611..9405
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8611..9405
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8665..8684)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9275..9294
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9593..9612)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9637..9684
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10013..10601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10502..10521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtatcgat
      661 gccgccacca tggctgcagt gaccatgtcc gtgtctggga ggaaggtagc ctccaggcca
      721 ggcccggtgc ctgaggcagc ccaatcgttc ctctacgcgc cccggacgcc aaatgtaggt
      781 ggccctggag ggccacaggt ggagtggaca gcccggcgca tggtgtgggt gccctcggaa
      841 ctgcatgggt tcgaggcagc agccctgcgg gatgaagggg aggaggaggc agaagtggag
      901 ctggcggaga gtgggcgccg cctgcggctg cccagggacc agatccagcg catgaaccca
      961 cccaagttca gcaaggcaga agatatggct gagctcacct gcctcaacga ggcctcggtc
     1021 ctgcacaacc tgcgagaacg ctactactcc gggctcattt atacctactc tggcctcttc
     1081 tgtgtggtca ttaacccata caagcagctg cccatctaca cggaggccat tgttgaaatg
     1141 taccggggca agaagcgcca tgaggtgcca cctcacgtgt atgctgtgac ggagggcgcg
     1201 taccgcagca tgcttcagga tcgtgaggat caatccattc tctgcacggg agagtctggc
     1261 gctgggaaga cggagaacac caagaaggtc atccagtacc tggcccatgt ggcatcatct
     1321 ccaaagggca ggaaggagcc tggtgtccct ggggagctag agcgtcagct tcttcaagcc
     1381 aaccccatcc tagaggcctt tggcaatgcc aagacagtga agaacgacaa ctcttcccga
     1441 tttggcaaat tcatccgcat caactttgat attgctggct acatcgtggg agcaaacatc
     1501 gagacctatc tgttggagaa gtcccgggcc atcagacagg ccaaggatga atgcagcttc
     1561 catatcttct accagctgct agggggcgct ggggagcagc taaaagctga cctccttctg
     1621 gagccctgtt cccattatcg cttcctgacc aatgggccct catcgtcccc gggccaggag
     1681 cgtgagttat tccaggagac cctggagtcc ctgcgtgtgc tgggcctcct cccagaagag
     1741 atcactgcca tgctgcgcac tgtctctgct gtcctccagt ttggcaacat tgtcctgaag
     1801 aaagagcgca atacggacca agccaccatg cctgacaaca cagctgccca gaagctttgc
     1861 cgcctcttgg gactcggagt gaccgacttc tccagagccc ttctcacacc ccgcatcaaa
     1921 gtgggccgag attatgttca gaaagcacaa accaaggagc aggctgactt tgcgctggag
     1981 gctctggcca aagctaccta tgagcgcctg ttccgctggc tggttctgcg gctcaaccgt
     2041 gccctggaca gaagcccgcg gcagggtgcc tccttcctgg gcatcctgga catcgcgggc
     2101 tttgagatct tccagctgaa ctccttcgag cagctgtgca tcaactacac caacgagaag
     2161 ctacagcagc tattcaacca caccatgttc gtgctggagc aggaggagta ccagcgagag
     2221 ggcatcccct ggaccttcct agacttcggg ttggacctgc aaccttgcat cgacctcatt
     2281 gagcgtccgg ccaaccctcc aggtctcctg gccctgctgg acgaggagtg ctggttcccc
     2341 aaggccacgg acaagtcttt tgtggagaag gtcgcccagg agcagggcag ccaccccaaa
     2401 ttccagcgcc ccaggaacct gcgagatcag gccgacttca gcgtcctgca ctacgccggc
     2461 aaggttgact acaaagccag tgagtggctg atgaagaaca tggacccact gaatgacaat
     2521 gtggccgcct tgcttcacca gagcacggat cgtctcacag ctgagatctg gaaggatgtg
     2581 gagggcatcg tggggctgga gcaagtaagc agccttggag atggcccacc gggaggccgc
     2641 ccccgccgtg gaatgttccg gactgtgggg cagctctaca aagaatccct gagccgcctc
     2701 atggccacgc tcagcaacac caaccctagt tttgtccgct gcatcgttcc caatcatgag
     2761 aagagggctg gaaagctgga gccgcgcctg gtgctggacc aactccgttg taacggggtc
     2821 ctcgagggta tacgcatctg tcgccaaggc ttccccaacc gcatcctctt ccaggagttc
     2881 cgacagcgct atgaaatcct caccccgaac gctattccca agggcttcat ggacggcaaa
     2941 caggcctgtg agaagatgat ccaggccctg gagctagacc ccaacctgta ccgtgttggc
     3001 caaagcaaga tcttcttccg ggcaggggtc ctggcccagc tggaggagga gcgggacctg
     3061 aaagtcaccg acatcatagt gtctttccag gcagcggcac ggggctacct ggcccgtagg
     3121 gctttccaga gacggcagca gcagcagagt gctctgaggg tgatgcagag aaactgtgct
     3181 gcctacctca agctcaggaa ctggcagtgg tggaggctgt tcatcaaggt gaagcccctg
     3241 ctgcaggtga cacggcagga tgaggtgctg caggcgcgcg cccaggagct gcagaaagtt
     3301 caggagctgc agcagcagag cgctcgtgaa gtgggggaac tgcagggtcg agtggcacag
     3361 ctagaggagg agcgcacgcg cctggctgag cagcttcgag cagaagccga gctctgctct
     3421 gaggccgagg agacgcgggc gcgactggct gcccggaagc aggagctgga gctggtggtg
     3481 acagagctgg aggcacgagt gggcgaggaa gaagagtgca gccggcagct gcagagtgag
     3541 aagaagaggc tgcagcagca tatccaggag ctagagagcc acctggaagc tgaggagggt
     3601 gcccggcaga agctacagct ggagaaggtg accacagagg ccaagatgaa gaaatttgag
     3661 gaggacctgc tgctcctgga ggaccagaat tccaagctga gcaaggagcg gaggctgctg
     3721 gaggagcggc tggctgagtt ctcctcacag gcagcagaag aggaagagaa agtcaaaagt
     3781 ctcaacaagc tgaggctcaa atatgaagcc acaatctcag acatggaaga ccggctgaag
     3841 aaggaggaga agggacgcca ggaactagag aagctgaagc gacggctgga cggggagagc
     3901 tcagagcttc aggagcagat ggtggagcag aagcagaggg cagaggaact gctcgcacag
     3961 ctgggccgca aggaggatga gctgcaggcc gccctgctca gggcagagga agagggtggt
     4021 gcccgtgccc agttgctcaa gtccctgcga gaggcacagg ctggccttgc tgaggctcag
     4081 gaggacctgg aagctgagcg ggtagccagg gccaaggcgg agaagcagcg ccgggacctg
     4141 ggcgaggagt tggaggccct acgtggggag ctcgaggaca ctctggattc caccaacgcc
     4201 cagcaggagc tgcggtccaa gagggagcag gaggtgacag agctgaagaa agcattggaa
     4261 gaggagtccc gtgcccatga ggtgtccatg caggagctga gacagaggca tagccaggca
     4321 ctggtggaga tggccgagca gttggagcaa gcccggaggg gcaaaggtgt gtgggagaag
     4381 actcggctat ccctggaggc tgaggtgtcc gagctgaagg ccgagctgag cagcctgcag
     4441 acctcgagac aggagggtga gcagaagagg cgccgcctgg agtcccagct acaggaggtc
     4501 cagggccgat ccagtgattc ggagcgggct cggtctgagg ctgctgagaa gctgcagaga
     4561 gcccaggcgg aacttgagag cgtgtccaca gccctgagtg aggcggagtc caaagccatc
     4621 aggctgggca aggagctgag cagtgcagag tcccagctgc atgacaccca ggaactgctt
     4681 caggaggaga ccagggcaaa gctggccttg gggtcccgtg tgcgtgccct agaggccgag
     4741 gcggcggggc ttcgggagca gatggaagag gaggtggttg ccagggaacg ggctggccgg
     4801 gagctgcaga gcacgcaggc ccagctctct gaatggcggc gccgccagga agaagaggct
     4861 gcggtgctgg aggctgggga ggaggctcgg cgccgtgcag cccgggaggc agagaccctg
     4921 acccagcgcc tggcagaaaa gactgaggct gtagaacgac tggagcgagc ccggcgccga
     4981 ctgcagcagg agttggacga tgccactgtg gatctggggc agcagaagca gctcctgagc
     5041 acactggaga agaagcagcg gaaatttgac cagctcctgg cagaggagaa ggctgcagtt
     5101 ctacgggctg tggaagaccg tgaacggata gaggccgaag gccgggagcg agaggcccgg
     5161 gccctgtcgc tgacccgggc cctggaagag gagcaggagg cccgggagga gctggagagg
     5221 cagaaccgtg ctctgagggc tgagctggaa gcactgctga gcagcaagga tgacgtgggc
     5281 aagaacgtgc acgagctgga gcgagcccgt aaggcggctg aacaggcagc cagtgacctg
     5341 cggacacagg tgacagaatt ggaggatgag ctgacagccg cagaggatgc caagctgcgc
     5401 ctggaggtga ctgtgcaggc tctgaaggct caacatgaac gcgacctgca gggccgcgat
     5461 gatgccggtg aggagaggcg gaggcagctg gccaagcagc taagagacgc agaggtagag
     5521 cgcgatgagg aacggaagca gagggcactg gctatggctg cccgcaagaa gctggagctg
     5581 gaactggagg agttgaaggc gcagacatct gctgctgggc agggcaagga agaggcagtg
     5641 aagcagctga agaagatgca ggtccagatg aaggagctgt ggcgggaggt agaggagacg
     5701 cgtagctccc gcgacgagat gtttaccctg agcagggaaa atgagaagaa gctcaagggg
     5761 ctggaagctg aggtgctgcg tctgcaagag gaacttgctg cctcagaccg agcccggagg
     5821 caggcccagc aagacagaga cgagatggca gaggaggtgg ccagtggcaa tcttagcaag
     5881 gcagccaccc tggaggaaaa acggcagctg gaggggcgac tgagccagtt ggaagaggag
     5941 ctggaggaag aacagaacaa ctcggagctg ctcaaggacc attaccgaaa gctagtgcta
     6001 caggtcgagt ccctcaccac agaactgtct gccgaacgaa gtttctcagc caaggccgag
     6061 agtggacggc agcagctgga gcggcagatc caggaactgc gggcccgctt gggtgaagag
     6121 gatgctggag cccgagccag gcagaaaatg ctgatcgctg ctctggagtc taaactggcc
     6181 caggcagagg agcagctgga gcaggagagc agggagcgca tcctctctgg caagctggta
     6241 cgcagagctg agaagcggct gaaggaggta gttcttcagg tggatgaaga gcgcagggtg
     6301 gctgaccagg tccgggacca gctggagaaa agcaacctcc ggctgaagca gctcaagagg
     6361 cagctggagg aggcagagga ggaggcatct cgggcacagg ctggtcggag gcggctgcag
     6421 cgggagctgg aggacgtcac tgagtctgca gaatccatga accgggaggt gaccacgctg
     6481 aggaacaggc tccggcgtgg cccacttaca ttcaccacac ggactgtgcg ccaggtgttc
     6541 cggctggaag agggcgtggc ttctgacgag gaagaggctg aaggagctga acctggctct
     6601 gcaccaggcc aggagccgga ggctccgccc cctgccacac cccaaggatc catcgccacc
     6661 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6721 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6781 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6841 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6901 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6961 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     7021 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     7081 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     7141 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     7201 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     7261 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     7321 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     7381 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     7441 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     7501 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7561 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7621 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7681 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7741 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7801 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7861 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7921 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7981 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     8041 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     8101 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8161 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     8221 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     8281 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     8341 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8401 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8461 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8521 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     8581 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     8641 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     8701 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     8761 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     8821 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     8881 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     8941 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     9001 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     9061 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     9121 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     9181 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     9241 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     9301 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     9361 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     9421 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     9481 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     9541 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     9601 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     9661 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     9721 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     9781 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     9841 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     9901 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9961 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10021 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10081 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10141 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10201 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10261 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10321 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10381 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10441 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10501 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10561 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10621 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10681 ctgattctgt ggataaccgt attaccgc
//
