LOCUS       sequence-151439-        7827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Hollenberg SM, Cheng PF, Weintraub H
  TITLE     Use of a conditional MyoD transcription factor in studies of MyoD 
            trans-activation and muscle determination.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Sep 1. 90(17):8028-32.
  PUBMED    8396258
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1993 Sep 1. 90(17):8028-32."
COMMENT     Sequence Label: pBABE MDER
FEATURES             Location/Qualifiers
     source          1..7827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(810..1409)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(893..913)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1390..1409
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1419..1748)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1433..1568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1733..1753
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(2893..3783)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     CDS             complement(2896..3831)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             complement(4552..4968)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(4568..4584)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4600..4622)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5169..5368
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             5433..5902
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5933..6037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6038..6106,6107..6898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6256..6275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7058..7646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7547..7566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7800..7817
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgatgcat ggggtcgtgc gctcctttcg
      781 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
      841 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
      901 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
      961 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     1021 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     1081 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     1141 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     1201 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     1261 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     1321 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     1381 aggcgcaccg tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc taggcctcca
     1441 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     1501 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1561 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1621 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     1681 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     1741 tgacacacat tccacagggt cgaccactgt gctggcgaat tcggatttcc aacacctgac
     1801 tcgcccgctt gaggaataaa catacaaagt ttttgttgca ctacacagca tgcctgggag
     1861 ataaatatag cccaaggggc tcccgctctg gccaagcaac tcttatttac acgaatagat
     1921 tacaaaagtc ttatataaaa aaataagtgg tcctgaaccg cgggaagggg gagagtgggg
     1981 tatggttaca cctgttacac ctgagacctg agtggcctcc gcaagctgtg gggaaaagtg
     2041 ggtgccagga gggctccaga aagtgacaaa caaaaggatg tggagggcag ggcttaggtg
     2101 tgcaaggctc acccctgtgc tatgaggaaa ggaagagcgc ctggccactc aaggatcagc
     2161 tctggcccag tgaggtgcag ccagagtgca agtggccttc gctgtgagcc gcatgaaaaa
     2221 acaaacaaac aactgaagga ctacaacaac aacaacaaaa aggactatgt cctttctttg
     2281 gggctggatc taggacagac ttctgctctt cccttccctc tggaagaacg gcttcgaaag
     2341 gacagttggg aagagtgtca tttaagcttc atcttttggg cgtgaagaac caggggcacc
     2401 atccccagga gtgcctacgg tggtgcgccc tctgctgctg cagtcgatct ctcaaagcac
     2461 ctgataaatc gcattggggt ttgagcctgc aggacactga ggggcggcgt cgggagacgg
     2521 ggtctgggtt ccctgttctg tgtcgcttag ggatgccccc tctggcggac ccggaggcga
     2581 ctctggtggt gcatctgcca aaagcagcgc aggcgcagcg gggctgtctg tggagatgcg
     2641 ctccactatg ctggacaggc agtcgaggct cgacacagcc gcactcttcc ctggcctgga
     2701 ctcgcgcacc gcctcactgt agtaggcggt gtcgtagcca ttctgccgcc gggggccgct
     2761 tggggggccg ctgtaatcca tcatgccatc agagcagttg gagcgcgggc tggacgcgtc
     2821 tgagtcgcca ctgtagtgct cgctgccacg gcctgggggc agcggtccag gtgcgtagaa
     2881 ggcagcggcg atgactgtgg cagggaaacc ctctgcctcc cccgtgatgt aatacttttg
     2941 caaggaatgc gatgaagtag agcccgcagt ggccaagtgg ctttggtccg tctcctccac
     3001 ggatgcccct ccacggctag tgggcgcatg taggcggtgg gcgtccagca tctccagcag
     3061 caggtcatag aggggcacca cgttcttgca cttcatgctg tacagatgct ccatgccttt
     3121 gttactcatg tgcctgatgt gggagaggat gaggaggagc tgggccagcc gctggtgctg
     3181 ctgctgcagg gtcaggcctg ccttggccat caggtggatc aaagtgtctg tgatcttgtc
     3241 caggactcgg tggatatggt ccttctcttc cagagacttc agggtgctgg acagaaatgt
     3301 gtacactcca gaattaagca aaataataga tttgaggcac acaaactcct ctccctgcag
     3361 attcatcatg cggaaccgag atgatgtagc cagcagcatg tcgaagatct ccaccatgcc
     3421 ctctacacat tttccctggt tcctgtccaa gagcaagtta ggagcaaaca gtagcttcac
     3481 tgggtgctcc atggagcgcc agacgagacc aatcatcagg atctctagcc aggcacattc
     3541 tagaaggtgg acctgatcat ggagggtcaa atccacaaag cctggcaccc tcttcgccca
     3601 gttgatcatg tgaaccagct ccctgtctgc caggttggtc agtaagccca tcatcgaagc
     3661 ttcactgaag ggtctggtag gatcatactc ggaatagagt atggggggct cagcatccaa
     3721 caaggcactg accatctggt cggccgtcag ggacaaggcc aggctgttct tcttagagcg
     3781 tttgatcatg agcgggcttg gccaaaggtt ggcagctctc atgtctccag cagaatcgcc
     3841 agggggcgcg gcgtcctggt cgcgcagcag agcctgcaga ccttcgatgt agcggatggc
     3901 gttgcgcagg atctccacct tgggtagccg ctggttcggg ttgctggacg tgcagcgctt
     3961 gagcgtctcg aaggcctcat tcactttgct caggcggcgg cgctcgcgca tggtggcggc
     4021 cttgcggcga tcagcgttgg tggtcttgcg cttgcacgcc ttgcaggccc acagcaagca
     4081 gcgacccgcc tggtggtgcc cgctgggcgc gcgcacatgc tcatcctcac gagcgcctgg
     4141 gcctgggtgc accgcagtag agaagtgtgc gtgctcctcc ggtttcagga gggctcccac
     4201 gtgcaccagg cgcgggtcca ggtcctcaaa aaagcgcagg tctggtgagt cgaaacacgg
     4261 atcatcatag aagtcgtctg ctgtctcaaa ggagcagaga gagccgtcgg ggcctgtcaa
     4321 gtctatgtcc cggagtggcg gcgatagaag ctccatatcc cagttcctgg gtccagcctc
     4381 aacccaagcc gtgagagtcg tcttaacttt ctgccactcc ggaaccccaa cagtacaatg
     4441 acaaaggttc tgtgggttgg aatgcacacc ggctgtcctc tacccctcct ccctgtcctg
     4501 tcctgtcaga ggtgtccgaa ttcctacgta ccaccacact gggatccggc cggcgcctag
     4561 agaaggagtg agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag
     4621 ggggagagac ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt
     4681 gacccaggga ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc
     4741 tgggtgtcca tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg
     4801 gttaaaggtg ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa
     4861 ggtaacccaa cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc
     4921 tttccagtga cctaaggtca aacttaaggg agtggtaaca gtctggccct aattttcaga
     4981 caaatacaga aacacagtca gacagagaca acacagaacg atgctgcagc gctgcagcag
     5041 acaagacgcg cggcttcggt tccaaaccga aagcaaaaat tcagacggag gcgggaactg
     5101 ttttaggttc tcgtctccta ccagaaccac atatcctgac ggggtcggat tccacatcga
     5161 ctcccttcct caggtcgggc cacaaaaacg gcccccaaag tccctgggac gtctcccagg
     5221 gttgcggccg ggtgttcaga actcgtcagt tccaccacgg gtccgccaga tacagagcta
     5281 gttagctaac tagtaccgac gcaggcgcat aaaatcagtc atagacacta gacaatcgga
     5341 cagacacaga taagttgctg gccagcttgc ctcccggtgg tgggtcggtg gtccctgggc
     5401 aggggtctcc cgatcccgga cgagccccca aatgaaagac ccccgctgac gggtagtcaa
     5461 tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc aactgcaaga
     5521 gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact ggcgccccga
     5581 gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga
     5641 gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac
     5701 cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat ctggggacca
     5761 tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca
     5821 tattcagctg ttccatctgt tcttggccct gagccggggc aggaactgct taccacagat
     5881 atcctgtttg gcccatattc aggctgcagg tggcactttt cggggaaatg tgcgcggaac
     5941 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6001 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6061 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6241 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6421 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6901 gtcagaccaa gtttactcat atatacttta gattgatttg cggccgcaaa aggatctagg
     6961 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7021 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7081 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7141 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7201 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7261 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7321 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7381 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7441 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7501 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7561 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7621 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7681 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7741 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7801 gcgagtcagt gagcgaggaa gcggaag
//
