LOCUS       sequence-151446-        9227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized dead (832A) LbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9227)
  AUTHORS   Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, 
            Fodor E, Welker E
  TITLE     Cpf1 nucleases demonstrate robust activity to induce DNA 
            modification by exploiting homology directed repair pathways in 
            mammalian cells.
  JOURNAL   Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
  PUBMED    27630115
REFERENCE   2  (bases 1 to 9227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Direct."; date: "2016-09-14"; volume: "11:46. doi"; pages: " 
            10.1186/s13062-016-0147-0"
COMMENT     Sequence Label: WN10151
FEATURES             Location/Qualifiers
     source          1..9227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             111..3794
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             3795..3842
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3849..3929
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4003..4020)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4009..4233
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4279..4707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4366..4385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4576..4597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4716..4736)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4721..5050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4901..5036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4963..4982
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5117..5911
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5171..5190)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5781..5800
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6085..6206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6122..6141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6176..6195
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6255..6271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6255..6271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6268..6290)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6279..6295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6303..6309,6310..6327,6328..6333))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6348..6369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6486..6503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6657..7242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6737..6756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7413..8204,8205..8273))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8036..8055
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8274..8378)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8453..8472)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8644..9023
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9024..9227
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9178..9198
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgccacc atgagcaagc
      121 tggagaagtt tacaaactgc tactccctgt ctaagaccct gaggttcaag gccatccctg
      181 tgggcaagac ccaggagaac atcgacaata agcggctgct ggtggaggac gagaagagag
      241 ccgaggatta taagggcgtg aagaagctgc tggatcgcta ctatctgtct tttatcaacg
      301 acgtgctgca cagcatcaag ctgaagaatc tgaacaatta catcagcctg ttccggaaga
      361 aaaccagaac cgagaaggag aataaggagc tggagaacct ggagatcaat ctgcggaagg
      421 agatcgccaa ggccttcaag ggcaacgagg gctacaagtc cctgtttaag aaggatatca
      481 tcgagacaat cctgccagag ttcctggacg ataaggacga gatcgccctg gtgaacagct
      541 tcaatggctt taccacagcc ttcaccggct tctttgataa cagagagaat atgttttccg
      601 aggaggccaa gagcacatcc atcgccttca ggtgtatcaa cgagaatctg acccgctaca
      661 tctctaatat ggacatcttc gagaaggtgg acgccatctt tgataagcac gaggtgcagg
      721 agatcaagga gaagatcctg aacagcgact atgatgtgga ggatttcttt gagggcgagt
      781 tctttaactt tgtgctgaca caggagggca tcgacgtgta taacgccatc atcggcggct
      841 tcgtgaccga gagcggcgag aagatcaagg gcctgaacga gtacatcaac ctgtataatc
      901 agaaaaccaa gcagaagctg cctaagttta agccactgta taagcaggtg ctgagcgatc
      961 gggagtctct gagcttctac ggcgagggct atacatccga tgaggaggtg ctggaggtgt
     1021 ttagaaacac cctgaacaag aacagcgaga tcttcagctc catcaagaag ctggagaagc
     1081 tgttcaagaa ttttgacgag tactctagcg ccggcatctt tgtgaagaac ggccccgcca
     1141 tcagcacaat ctccaaggat atcttcggcg agtggaacgt gatccgggac aagtggaatg
     1201 ccgagtatga cgatatccac ctgaagaaga aggccgtggt gaccgagaag tacgaggacg
     1261 atcggagaaa gtccttcaag aagatcggct ccttttctct ggagcagctg caggagtacg
     1321 ccgacgccga tctgtctgtg gtggagaagc tgaaggagat catcatccag aaggtggatg
     1381 agatctacaa ggtgtatggc tcctctgaga agctgttcga cgccgatttt gtgctggaga
     1441 agagcctgaa gaagaacgac gccgtggtgg ccatcatgaa ggacctgctg gattctgtga
     1501 agagcttcga gaattacatc aaggccttct ttggcgaggg caaggagaca aacagggacg
     1561 agtccttcta tggcgatttt gtgctggcct acgacatcct gctgaaggtg gaccacatct
     1621 acgatgccat ccgcaattat gtgacccaga agccctactc taaggataag ttcaagctgt
     1681 attttcagaa ccctcagttc atgggcggct gggacaagga taaggagaca gactatcggg
     1741 ccaccatcct gagatacggc tccaagtact atctggccat catggataag aagtacgcca
     1801 agtgcctgca gaagatcgac aaggacgatg tgaacggcaa ttacgagaag atcaactata
     1861 agctgctgcc cggccctaat aagatgctgc caaaggtgtt cttttctaag aagtggatgg
     1921 cctactataa ccccagcgag gacatccaga agatctacaa gaatggcaca ttcaagaagg
     1981 gcgatatgtt taacctgaat gactgtcaca agctgatcga cttctttaag gatagcatct
     2041 cccggtatcc aaagtggtcc aatgcctacg atttcaactt ttctgagaca gagaagtata
     2101 aggacatcgc cggcttttac agagaggtgg aggagcaggg ctataaggtg agcttcgagt
     2161 ctgccagcaa gaaggaggtg gataagctgg tggaggaggg caagctgtat atgttccaga
     2221 tctataacaa ggacttttcc gataagtctc acggcacacc caatctgcac accatgtact
     2281 tcaagctgct gtttgacgag aacaatcacg gacagatcag gctgagcgga ggagcagagc
     2341 tgttcatgag gcgcgcctcc ctgaagaagg aggagctggt ggtgcaccca gccaactccc
     2401 ctatcgccaa caagaatcca gataatccca agaaaaccac aaccctgtcc tacgacgtgt
     2461 ataaggataa gaggttttct gaggaccagt acgagctgca catcccaatc gccatcaata
     2521 agtgccccaa gaacatcttc aagatcaata cagaggtgcg cgtgctgctg aagcacgacg
     2581 ataaccccta tgtgatcggc atcgcgaggg gcgagcgcaa tctgctgtat atcgtggtgg
     2641 tggacggcaa gggcaacatc gtggagcagt attccctgaa cgagatcatc aacaacttca
     2701 acggcatcag gatcaagaca gattaccact ctctgctgga caagaaggag aaggagaggt
     2761 tcgaggcccg ccagaactgg acctccatcg agaatatcaa ggagctgaag gccggctata
     2821 tctctcaggt ggtgcacaag atctgcgagc tggtggagaa gtacgatgcc gtgatcgccc
     2881 tggaggacct gaactctggc tttaagaata gccgcgtgaa ggtggagaag caggtgtatc
     2941 agaagttcga gaagatgctg atcgataagc tgaactacat ggtggacaag aagtctaatc
     3001 cttgtgcaac aggcggcgcc ctgaagggct atcagatcac caataagttc gagagcttta
     3061 agtccatgtc tacccagaac ggcttcatct tttacatccc tgcctggctg acatccaaga
     3121 tcgatccatc taccggcttt gtgaacctgc tgaaaaccaa gtataccagc atcgccgatt
     3181 ccaagaagtt catcagctcc tttgacagga tcatgtacgt gcccgaggag gatctgttcg
     3241 agtttgccct ggactataag aacttctctc gcacagacgc cgattacatc aagaagtgga
     3301 agctgtactc ctacggcaac cggatcagaa tcttccggaa tcctaagaag aacaacgtgt
     3361 tcgactggga ggaggtgtgc ctgaccagcg cctataagga gctgttcaac aagtacggca
     3421 tcaattatca gcagggcgat atcagagccc tgctgtgcga gcagtccgac aaggccttct
     3481 actctagctt tatggccctg atgagcctga tgctgcagat gcggaacagc atcacaggcc
     3541 gcaccgacgt ggattttctg atcagccctg tgaagaactc cgacggcatc ttctacgata
     3601 gccggaacta tgaggcccag gagaatgcca tcctgccaaa gaacgccgac gccaatggcg
     3661 cctataacat cgccagaaag gtgctgtggg ccatcggcca gttcaagaag gccgaggacg
     3721 agaagctgga taaggtgaag atcgccatct ctaacaagga gtggctggag tacgcccaga
     3781 ccagcgtgaa gcacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa
     3841 agggatccta cccatacgat gttccagatt acgcttatcc ctacgacgtg cctgattatg
     3901 catacccata tgatgtcccc gactatgcct aagaattctg cagatatcca gcacagtggc
     3961 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4021 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4081 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4141 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4201 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4261 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4321 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4381 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4441 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4501 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4561 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4621 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4681 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4741 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4801 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4861 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4921 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4981 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5041 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     5101 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5161 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5221 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5281 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5341 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5401 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5461 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5521 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5581 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5641 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5701 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5761 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5821 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5881 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5941 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6001 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6061 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6121 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6181 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6241 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6301 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6361 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6421 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6541 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6601 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6661 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6721 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6781 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6841 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6901 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6961 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7021 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7081 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7141 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt
     7201 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7261 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7321 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7381 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7441 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7501 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7561 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7621 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7681 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7741 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7801 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7861 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7921 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7981 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8041 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8101 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8161 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8221 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8281 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8341 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8401 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     8461 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     8521 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     8581 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     8641 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     8701 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     8761 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     8821 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     8881 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     8941 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     9001 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     9061 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     9121 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     9181 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagct
//
