LOCUS       sequence-151457-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB094d
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(470..499)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(509..781)
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
ORIGIN
        1 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
       61 gatcaccaag gtagtcggca aataaacgcc atgggcatta aaaaatgccc tcttgggtta
      121 tcaagagggt cattatattt gctgaggagt gccgttaatt aagttagcag gtgcggtaat
      181 tgatactaat cggagaatac atgaacatgt aacccgcgct atcttcgccc catgcataaa
      241 ccgtgatggt gtaatccaca cccgggctca ggccactaat cgtcgcggta gagctactat
      301 acggaaccgt aaacgcctgc accgggctgt taccgcccgt ttcaccgtag gtgatacgat
      361 agtaatacac agagctacta gacatcgggg catcccaaga aatcagcagg ctcgtcgggg
      421 ttgctgcaac cacttccagt ttggtcggaa cgctactcac agagctgccc aagtcctctt
      481 cagaaataag cttttgttcc gcggccgcac gacgaccttc agcaatagcg gtaacggctt
      541 gtaattcagc ggcttcctgc tcttgctgtt cctggcgttc gcgaacgtcg aggatctggt
      601 tgttgatcag accttcttcg atttcgcgca gcgcgattac agtggtttta tcgttttctt
      661 ccggtaccag cggatccttt ccgcctacct gcatctgacg agcgcgacgc gcggcgacca
      721 gtaccaggtc aaaacggtta ccaattttct ctacagcgtc ctgaacagtt acgcgtgcca
      781 tttttttttc ctccttactg cacctgcagg taaaa
//
