LOCUS       sequence-151473-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB094b
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(469..498)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(508..780)
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
ORIGIN
        1 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
       61 atcaccaagg tagtcggcaa ataaacgcca tgggcattaa aaaatgccct cttgggttat
      121 caagagggtc attatatttg ctgaggagtg ccgttaatta agttagcagg tgcggtaatt
      181 gatactaatc ggagaataca tgaacatgta acccgcgcta tcttcgcccc atgcataaac
      241 cgtgatggtg taatccacac ccgggctcag gccactaatc gtcgcggtag agctactata
      301 cggaaccgta aattcctgca ccgggctgtt accgcccgtt tcaccgtagg tgatacgata
      361 gtacgccaca gagctactag acatcggggc atcccaagaa atcagcaggc tcgtcggggt
      421 tgctgcaacc acttccagtt tggtcggaac gctactcaca gagctgccca agtcctcttc
      481 agaaataagc ttttgttccg cggccgcacg acgaccttca gcaatagcgg taacggcttg
      541 taattcagcg gcttcctgct cttgctgttc ctggcgttcg cgaacgtcga ggatctggtt
      601 gttgatcaga ccttcttcga tttcgcgcag cgcgattaca gtggttttat cgttttcttc
      661 cggtaccagc ggatcctttc cgcctacctg catctgacga gcgcgacgcg cggcgaccag
      721 taccaggtca aaacggttac caattttctc tacagcgtcc tgaacagtta cgcgtgccat
      781 ttttttttcc tccttactgc acctgcaggt aaaaaaacgg gtatggagaa acagtagaga
      841 gttgcgataa aa
//
