LOCUS       sequence-151480-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Output plasmid: contains 5-state register that regulates expression 
            of GFP, RFP, and BFP. Expresses a unique fluorescent signature in 
            each state..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR291
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    314..362
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
       61 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
      121 gtggccagat actgcgacct ccctagcaaa ctggggcact aatgtgaaaa agcccgcgca
      181 agcgggtttt tttatgtctc agctaggtgt agttgcgcta acaccgtgcg tttgactatt
      241 ttacctctgg cggtgataat ggttgcatat agtgaacaag gattaacgcc gatcgtccgt
      301 gacattctgt gcgcggccgg cttgtcgacg acggcggtct ccgtcgtcag gatcatccgg
      361 gccagcggct gtctatgcca gaagtagaac atagcgcccc acgtggggag actaaggcag
      421 ccagatggct gcctttttta caggtgccag gtgatttcaa ctaataaaaa aaaccccggg
      481 acctttcggt gcgggggtct tactatcccc taaacttcaa atcacaaaat aaaaaggccg
      541 gttaaaccga ccttttactc gttcttgtcc gcacaggtgg atgcttatta agcaccggtg
      601 gagtgacgac cttcagcacg ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag
      661 gtgatgtcca gtttgatgtc ggttttgtaa gcacc
//
