LOCUS       sequence-151489-       11854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG inducible expressoin of sucrose phosphate synthase derived from
            Synechococcus elongatus PCC 7942.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Abramson BW, Kachel B, Kramer DM, Ducat DC
  TITLE     Increased Photochemical Efficiency in Cyanobacteria via an 
            Engineered Sucrose Sink.
  JOURNAL   Plant Cell Physiol. 2016 Oct 13. pii: pcw169.
  PUBMED    27742883
REFERENCE   2  (bases 1 to 11854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2016 Oct 13. pii: pcw169."
COMMENT     Sequence Label: SPS-7942
FEATURES             Location/Qualifiers
     source          1..11854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(51..78)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(170..256)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(705..724)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             716..1531
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             716..1531
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(789..808)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     complement(2634..2653)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             4115..4306
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    4408..4548
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4463..4482
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4563..4580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4734..5322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4814..4833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5493..6284,6285..6353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6116..6135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6354..6458)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6526..6544
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8200..8277
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8278..9360
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8297..8316)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(9592..9597,9598..9614,9615..9621)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    9629..9645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctagcaagct tggccggatc cggccggatc cgggagtttg tagaaacgca aaaaggccat
       61 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
      121 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
      181 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      241 cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
      301 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
      361 ctactgccgc caggcaaatt ctgttttatt gagccgttac cccacctact agctaatccc
      421 atctgggcac atccgatggc aagaggcccg aaggtccccc tctttggtct tgcgacgtta
      481 tgcggtatta gctaccgttt ccagtagtta tccccctcca tcaggcagtt tcccagacat
      541 tactcacccg tccgccactc gtcagcaaag aagcaagctt agatcgacct gcaggggggg
      601 gggggaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
      661 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
      721 ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga
      781 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
      841 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
      901 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
      961 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1021 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1081 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1141 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1201 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1261 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1321 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     1381 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     1441 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     1501 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     1561 gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg aacttttgct
     1621 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
     1681 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
     1741 actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca
     1801 cgaggcagac ctcagcgccc ccccccccct gcaggtcgat ctggtaaccc cagcgcggtt
     1861 gctaccaagt agtgacccgc ttcgtgatgc aaaatccgct gacgatattc gggcgatcgc
     1921 tgctgaatgc catcgagcag taacgtggca ccccgcccct gccaagtcac cgcatccaga
     1981 ctgaacagca ccaagaggct aaaacccaat cccgccggta gcagcggaga actacccagc
     2041 attggtccca ccaaagctaa tgccgtcgtg gtaaaaatcg cgatcgccgt cagactcaag
     2101 cccagttcgc tcatgcttcc tcatctaggt cacagtcttc ggcgatcgca tcgatctgat
     2161 gctgcagcaa gcgttttcca taccggcgat cgcgccgtcg ccctttcgct gccgtggccc
     2221 gcttacgagc tcgtttatcg accacgatcg catccaaatc cgcgatcgct tcccagtccg
     2281 gcaattcagt ctggggcgtc cgtttcatta atcctgatca ggcacgaaat tgctgtgcgt
     2341 agtatcgcgc atagcggcca gcctctgcca acagcgcatc gtgattgcct gcctcaacaa
     2401 tctggccgcg ctccatcacc aagatgcggc tggcattacg aaccgtagcc agacggtgag
     2461 caatgataaa gaccgtccgt ccctgcatca cccgttctag ggcctcttgc accaaggttt
     2521 cggactcgga atcaagcgcc gaagtcgcct catccagaat taaaatgcgt ggatcctcta
     2581 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     2641 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     2701 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     2761 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     2821 aatgcaggag tcgcataagg gagagcgtcg atcgaccgat gcccttgaga gccttcaacc
     2881 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     2941 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     3001 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     3061 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     3121 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     3181 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     3241 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     3301 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt
     3361 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     3421 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     3481 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
     3541 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
     3601 ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat
     3661 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca
     3721 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac
     3781 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa
     3841 gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg
     3901 tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg
     3961 gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg
     4021 ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc
     4081 catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct
     4141 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
     4201 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
     4261 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     4321 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     4381 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     4441 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     4501 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     4561 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4621 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4681 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4741 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4801 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4861 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4921 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4981 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5041 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5101 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5161 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5221 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5281 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5341 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5401 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5461 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5521 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5581 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5641 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5701 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5761 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     5821 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5881 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5941 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6001 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6061 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     6121 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6181 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6241 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6301 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6361 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6421 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6481 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     6541 aggccctttc gtcttcaaga attagcttgt catctgccgg atgaggcaaa accctgccta
     6601 cggcgcgatt acatcgtccc agcgcgatcg ctcttactgt tgatggctcg tgcttaaaaa
     6661 caatgcaaac ttcaccgttt cagctggtga ttttcgactg tgatggtgtg cttgttgata
     6721 gcggaacgca tcactaatcg cgtctttgca gacatgctca atgaactggg tctgttggtg
     6781 actttggatg acatgtttga gcagtttgtg ggtcattcca tggctgactg tctcaaacta
     6841 attgagcgac ggttaggcaa tcctccaccc cctgactttg ttcagcacta tcaacgccgt
     6901 acccgtatcg cgttagaaac gcatctacaa gccgttcctg gggttgaaga ggctttggat
     6961 gctcttgaat tgccctactg tgttgcgtcc agtggtgatc atcaaaagat gcgaaccaca
     7021 ctgagcctga cgaagctctg gccacgattt gagggacgaa tcttcagcgt gactgaagta
     7081 cctcgcggca agccatttcc cgatgtcttt ttgttggccg ccgatcgctt cggggttaat
     7141 cctacggcct gcgctgtgat cgaagacacc cccttgggag tagcggcagg cgtggcggca
     7201 ggaatgcaag tgtttggcta cgcgggttcc atgcccgctt ggcgtctgca agaagccggt
     7261 gcccatctca tttttgacga tatgcgactg ctgcccagtc tgctccaatc gtcgccaaaa
     7321 gataactcca cagcattgcc caatccctaa cccctgctcg cgccgcaact acacactaaa
     7381 ccgttcctgc gcgatcgctc ttactgttga tggctcgtgc ttaaaaacaa tgcaacccta
     7441 accgtttcag ctggtgattt tcggacgatt tggcttacag ggataactga gagtcaacag
     7501 cctctgtccg tcattgcaca cccatccatg cactggggac ttgactcatg ctgaatcaca
     7561 tttcccttgt ccattgggcg agaggggagg ggaatcttct ggactcttca ctaagcggcg
     7621 atcgcaggtt cttctaccca agcagtggcg atcgcttgat tgcagtcttc aatgctggcc
     7681 tctgcagcca tcgccgccac caaagcatcg taggcgggac gttgttgctc cagtaaagtc
     7741 ttcgcccgta acaatcccca gcgactgcgt aaatccgctt cggcaggatt gcgatcgagt
     7801 tgccgccaca gttgtttcca ctgggcgcga tcgtcagctc ccccttccac gttgccgtag
     7861 accagttgct ctgccgctgc accggccatc aacacctgac accactgttc cagcgatcgc
     7921 tgactgagtt gcccctgtgc ggcttcggct tctagcgcag ctgcttggaa ctgcacaccc
     7981 ccgcgaccag gttgtccttg gcgcagcgct tcccacgctg agagggtgta gcccgtcacg
     8041 ggtaaccgat atctgtacac cgcggggtac cactagttac agacaagctg tgaccgtctc
     8101 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat
     8161 caattcgcgc gcgaaggcga agcggcatgc atttacgttg acaccatcga atggtgcaaa
     8221 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg
     8281 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     8341 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
     8401 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     8461 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     8521 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     8581 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     8641 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     8701 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     8761 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     8821 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     8881 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     8941 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     9001 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     9061 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     9121 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     9181 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     9241 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     9301 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9361 gcgcaacgca attaatgtga gttagcgcga attgatctgg tttgacagct tatcatcgac
     9421 tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg
     9481 caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg
     9541 ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt
     9601 aatcatccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaaggagg
     9661 aaaaaaaaat gtctagagtg gcagctcaaa atctctacat tctgcacatt cagacccatg
     9721 gtctgctgcg agggcagaac ttggaactgg ggcgagatgc cgacaccggc gggcagacca
     9781 agtacgtctt agaactggct caagcccaag ctaaatcccc acaagtccaa caagtcgaca
     9841 tcatcacccg ccaaatcacc gacccccgcg tcagtgttgg ttacagtcag gcgatcgaac
     9901 cctttgcgcc caaaggtcgg attgtccgtt tgccttttgg ccccaaacgc tacctccgta
     9961 aagagctgct ttggccccat ctctacacct ttgcggatgc aattctccaa tatctggctc
    10021 agcaaaagcg caccccgact tggattcagg cccactatgc tgatgctggc caagtgggat
    10081 cactgctgag tcgctggttg aatgtaccgc taattttcac agggcattct ctggggcgga
    10141 tcaagctaaa aaagctgttg gagcaagact ggccgcttga ggaaattgaa gcgcaattca
    10201 atattcaaca gcgaattgat gcggaggaga tgacgctcac tcatgctgac tggattgtcg
    10261 ccagcactca gcaggaagtg gaggagcaat accgcgttta cgatcgctac aacccagagc
    10321 gcaagcttgt cattccaccg ggtgtcgata ccgatcgctt caggtttcag cccttgggcg
    10381 atcgcggtgt tgttctccaa caggaactga gccgctttct gcgcgaccca gaaaaacctc
    10441 aaattctctg cctctgtcgc cccgcacctc gcaaaaatgt accggcgctg gtgcgagcct
    10501 ttggcgaaca tccttggctg cgcaaaaaag ccaaccttgt cttagtactg ggcagccgcc
    10561 aagacatcaa ccagatggat cgcggcagtc ggcaggtgtt ccaagagatt ttccatctgg
    10621 tcgatcgcta cgacctctac ggcagcgtcg cctatcccaa acagcatcag gctgatgatg
    10681 tgccggagtt ctatcgccta gcggctcatt ccggcggggt attcgtcaat ccggcgctga
    10741 ccgaaccttt tggtttgaca attttggagg caggaagctg cggcgtgccg gtggtggcaa
    10801 cccatgatgg cggcccccag gaaattctca aacactgtga tttcggcact ttagttgatg
    10861 tcagccgacc cgctaatatc gcgactgcac tcgccaccct gctgagcgat cgcgatcttt
    10921 ggcagtgcta tcaccgcaat ggcattgaaa aagttcccgc ccattacagc tgggatcaac
    10981 atgtcaatac cctgtttgag cgcatggaaa cggtggcttt gcctcgtcgt cgtgctgtca
    11041 gtttcgtacg gagtcgcaaa cgcttgattg atgccaaacg ccttgtcgtt agtgacatcg
    11101 acaacacact gttgggcgat cgtcaaggac tcgagaattt aatgacctat ctcgatcagt
    11161 atcgcgatca ttttgccttt ggaattgcca cggggcgtcg cctagactct gcccaagaag
    11221 tcttgaaaga gtggggcgtt ccttcgccaa acttctgggt gacttccgtc ggcagcgaga
    11281 ttcactatgg caccgatgct gaaccggata tcagctggga aaagcatatc aatcgcaact
    11341 ggaatcctca gcgaattcgg gcagtaatgg cacaactacc ctttcttgaa ctgcagccgg
    11401 aagaggatca aacacccttc aaagtcagct tctttgtccg cgatcgccac gagactgtgc
    11461 tgcgagaagt acggcaacat cttcgccgcc atcgcctgcg gctgaagtca atctattccc
    11521 atcaggagtt tcttgacatt ctgccgctag ctgcctcgaa aggggatgcg attcgccacc
    11581 tctcactccg ctggcggatt cctcttgaga acattttggt ggcaggcgat tctggtaacg
    11641 atgaggaaat gctcaagggc cataatctcg gcgttgtagt tggcaattac tcaccggaat
    11701 tggagccact gcgcagctac gagcgcgtct attttgctga gggccactat gctaatggca
    11761 ttctggaagc cttaaaacac tatcgctttt ttgaggcgat cgcttaacct tttcagttaa
    11821 agattgggcg atcgcgttgc tgcaataggt agcg
//
