LOCUS       sequence-151517-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses hygromycin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     empty vector
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLKO.1 hygro
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        120..368
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     120..140
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     291..310
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    431..548
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
       61 tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc cccttcaccg
      121 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      181 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      241 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      301 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      361 gaaacaccgg tccgcaggta tgcacgcgtg aattctcgac ctcgagacaa atggcagtat
      421 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      481 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      541 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg
      601 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
      661 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      721 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      781 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      841 gccgcacgtc tcactagtac cctc
//
