LOCUS       sequence-151519-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Broad range bacterial fluorescence labeling (ECFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Barbier M, Damron FH
  TITLE     Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.
  JOURNAL   PLoS One. 2016 Mar 3;11(3):e0146827. doi: 
            10.1371/journal.pone.0146827. eCollection 2016.
  PUBMED    26937640
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-3"; volume: "11(3):e0146827. doi"; pages: " 
            10.1371/journal.pone.0146827. eCollection 2016"
COMMENT     Sequence Label: pUCP30T-eCFP
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(17..19,20..22,23..736)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MLSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(62..83)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(323..342)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     670..691
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(747..764)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(747..763)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(756..778)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agaggagaaa ttaagcatgc tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
       61 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      121 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      181 cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta
      241 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      301 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      361 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      421 caacatcctg gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc
      481 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
      541 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      601 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
      661 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      721 cgagctgtac aagtaataag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
      781 aaccctggcg ttacccaact taatcgcctt gcagcacatc cc
//
