LOCUS       sequence-151521-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  246R. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses 
            GFP and RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP311
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(591..837)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(879..905)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cttgctcacc atgaattcgc gtttcgaata attagttgtt ttttgatctt ctcaagttgt
       61 cgttaaaagt cgttaaaatc aaaagcttgt caattggaac cagtcgcaat tatgaaagta
      121 agctaataat gatgataaaa aaaaaggttt aagacagggc agcttccttc tgtttatata
      181 ttgctgtcaa gtaggggtta ggcgcgccca tagggtatcc tcactccgat tgatgaagag
      241 acccttctgg ttccaatcct cactccgatt gatgtgacgt caatgggtgg agtatatcct
      301 cactccgatt gatgagtgct ctcgatgact tcgatatcct cactccgatt gatgaactcc
      361 gccccattga cgcaaatcct cactccgatt gatggtggaa cagcccaccc tgtacatcct
      421 cactccgatt gatgtgcggc ctcgagaagc agctgaatcc tcactccgat tgatgactgg
      481 accgtcacca attggagatc ctcactccga ttgatggtgg taaactcgca atatcctcac
      541 tccgattgat gaattgatct gccgcgatga cttctatatg atatgctagc tctcacttaa
      601 tcttctgtac tctgaagagg agtgggaaat accaagaaaa acatcaaact cgaatgattt
      661 tcccaaaccc ctaccacaag atattcatca gctgcgagat aggctgatca ggagcaagct
      721 cgtacgagaa gaaacaaaat gacaaaaaaa atcctatact atataggtta caaataaaaa
      781 agtatcaaaa atgaagcctg catctctcag gcaaatggca ttctgacatc ctcttgatta
      841 gaatctagca agaccggtct accgatggta cctcaagaag cgtagtccgg aacgtcgtac
      901 gggtagttaa ttaacatatc gagatcgaaa tcgtccagag catcggagcc ga
//
