LOCUS       sequence-151522-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim JS
  TITLE     Genome-wide analysis reveals specificities of Cpf1 endonucleases in 
            human cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609.
  PUBMED    27272384
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609."
COMMENT     Sequence Label: pMAL-his-LbCpf1-EC
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..43
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             113..133
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caacaacaat aacaataaca acaacctcgg gatcgaggga aggatttcac atatgcatca
       61 tcatcatcat catgagaacc tgtacttcca gggcgaattc ggctccggac ctccaaagaa
      121 aaagagaaaa gtaatgagca aactggaaaa atttacgaat tgttatagcc tgtccaagac
      181 cctgcgtttc aaagccatcc ccgttggcaa aacccaggag aatattgata ataaacgtct
      241 gctggttgag gatgaaaaaa gagcagaaga ctataaggga gtcaaaaaac tgctggatcg
      301 gtactacctg agctttataa atgacgtgct gcatagcatt aaactgaaaa atctgaataa
      361 ctatattagt ctgttccgca agaaaacccg aacagagaaa gaaaataaag agctggaaaa
      421 cctggagatc aatctgcgta aagagatcgc aaaagctttt aaaggaaatg aaggttataa
      481 aagcctgttc aaaaaagaca ttattgaaac catcctgccg gaatttctgg atgataaaga
      541 cgagatagcg ctcgtgaaca gcttcaacgg gttcacgacc gccttcacgg gctttttcga
      601 taacagggaa aatatgtttt cagaggaagc caaaagcacc tcgatagcgt tccgttgcat
      661 taatgaaaat ttgacaagat atatcagcaa catggatatt ttcgagaaag ttgatgcgat
      721 ctttgacaaa catgaagtgc aggagattaa ggaaaaaatt ctgaacagcg attatgatgt
      781 tgaggatttt ttcgaggggg aattttttaa ctttgtactg acacaggaag gtatagatgt
      841 gtataatgct attatcggcg ggttcgttac cgaatccggc gagaaaatta agggtctgaa
      901 tga
//
