LOCUS       sequence-151525-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express African green monkey pygerythrus TRIM5alpha in mammalan 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: CSII-IDR2-AGMpygTRIM5-FOS
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    46..98
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(102..125)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(132..155)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcggcttcg gccagtaacg ttaggggggg gggagggaga ggggcggatc ccgggcccgc
       61 ggtaccgtcg actgcagaat tcgaagcttg agctcgagtt atttttcgaa ctgcgggtgg
      121 ctccaagcgc tcttgtcatc gtcatccttg taatcgaatc cggatccggt accagagctt
      181 ggtgagcaca gagtcatggg gactgtacat tttctgggat ttaaatatgg aaatacaggc
      241 ttagaaaaag aacactgaga aaacttatag atgagaaatc catggtttgt gatattgaag
      301 aatgagacag tgcaagcctc atagtctacg aaaactccaa cacgatcagg acaaataatc
      361 acagagaggg gcacaatgaa aggagcaaaa ggagtatgtg aggaaccatc ctggaaagca
      421 ctatatttat ctccttcctg taaccctata acccagtagc catatttagg ttgataattt
      481 tcattttgtt caatattata cgttgcatcg ggttggaagc cagcacatac ccccaggatc
      541 caagcacttt tcttggacac atctacctcc cagtaatgtt tccctgatgt gatactttgg
      601 gaacccagga cgccagtaca ataattgaaa ttcgtgagtt tccctgatgt gatactttgg
      661 gagcccggga cgccagtaca ataactgaaa ttcgtgagtg acc
//
