LOCUS       sequence-151529-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline-dependent expression of ArchT-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Sakaguchi M, Kim K, Yu LM, Hashikawa Y, Sekine Y, Okumura Y, Kawano 
            M, Hayashi M, Kumar D, Boyden ES, McHugh TJ, Hayashi Y
  TITLE     Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the 
            Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice.
  JOURNAL   PLoS One. 2015 Jun 15;10(6):e0130163. doi: 
            10.1371/journal.pone.0130163. eCollection 2015.
  PUBMED    26075894
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-06-15"; volume: "10(6):e0130163. doi"; pages: " 
            10.1371/journal.pone.0130163. eCollection 2015"
COMMENT     Sequence Label: pTRE Tight ArchT-GFP
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             193..213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    638..759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     729..748
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    887..968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(979..996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
       61 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
      121 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
      181 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
      241 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
      301 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
      361 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
      421 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
      481 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
      541 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      601 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      661 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      721 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgac gtctctagag
      781 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      841 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      901 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      961 ttcactgcct cgagcttcct cgctcactga ctcgctgcgc tcggcgtcgt ggcggg
//
