LOCUS       sequence-151533-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline-dependent expression of ArchT-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Sakaguchi M, Kim K, Yu LM, Hashikawa Y, Sekine Y, Okumura Y, Kawano 
            M, Hayashi M, Kumar D, Boyden ES, McHugh TJ, Hayashi Y
  TITLE     Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the 
            Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice.
  JOURNAL   PLoS One. 2015 Jun 15;10(6):e0130163. doi: 
            10.1371/journal.pone.0130163. eCollection 2015.
  PUBMED    26075894
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-06-15"; volume: "10(6):e0130163. doi"; pages: " 
            10.1371/journal.pone.0130163. eCollection 2015"
COMMENT     Sequence Label: pTRE Tight ArchT-GFP
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..66
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    119..137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    190..208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        256..294
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     291..315
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             425..514
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="DSKGSSQKGSRLLLLLVVSNLLLCQGVVST"
ORIGIN
        1 ggcngnattn nccctatcag tggatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagaattcga gctcggtacc cggggatccg ccaccatggt
      361 cccgtgcacg ctgctcctgc tgttggcagc cgccctggct ccgactcaga cgcgggccag
      421 cgctgacagc aaaggttcgt cgcagaaagg gtcccgcctg ctcctgctgc tggtggtgtc
      481 aaatctactc ttgtgccagg gtgtggtctc cacccccgtc gctagcgacc ccatcgctct
      541 gcaggcagga tacgacctgc tgggcgacgg aaggccagag accctgtggc tgggaatcgg
      601 aaccctgctg atgctgatcg gcaccttcta cttcatcgtg aagggctggg gcgtgaccga
      661 caaggaggcc agggagtact acagcatcac aatcctggtg cccggcatcg ccagcgccgc
      721 ctacctgagc atgttcttcg gcatcggcct gaccgaggtg accgtggccg gcgaggtgct
      781 ggacatctac tacgccagat acgccgactg gctgttcacc acccctttgc ttctgctcga
      841 cctggccctg ctggctaagg tggacagggt gagcatcgga accctggtgg gagtggacgc
      901 cctgatgatc gtgaccggcc tgatcggcgc cctgagccac accccnactg gctaggtaca
      961 gctggtggct gttcagcacc atctgcatga tcgtggngct gnacttcctg gctaccagcc
     1021 tganggctgc tgctaaggaa aggggaacca aanggggnaa gcacctttaa nnccctgaac
     1081 cgcccctggg gntggggctg nggaccccct accccatcct ggggantaat cggaaaccna
     1141 ng
//
