LOCUS       sequence-151534-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline-dependent expression of ArchT-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Sakaguchi M, Kim K, Yu LM, Hashikawa Y, Sekine Y, Okumura Y, Kawano 
            M, Hayashi M, Kumar D, Boyden ES, McHugh TJ, Hayashi Y
  TITLE     Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the 
            Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice.
  JOURNAL   PLoS One. 2015 Jun 15;10(6):e0130163. doi: 
            10.1371/journal.pone.0130163. eCollection 2015.
  PUBMED    26075894
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-06-15"; volume: "10(6):e0130163. doi"; pages: " 
            10.1371/journal.pone.0130163. eCollection 2015"
COMMENT     Sequence Label: pTRE Tight ArchT-GFP
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..52
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    69..87
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    105..123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    176..194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    212..230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        242..280
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      336..345
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             411..500
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="DSKGSSQKGSRLLLLLVVSNLLLCQGVVST"
ORIGIN
        1 tatcagtgga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc
       61 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
      121 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct
      181 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      241 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga attcgagctc ggtacccggg gatccgccac catggtcccg tgcacgctgc
      361 tcctgctgtt ggcagccgcc ctggctccga ctcagacgcg ggccagcgct gacagcaaag
      421 gttcgtcgca gaaagggtcc cgcctgctcc tgctgctggt ggtgtcaaat ctactcttgt
      481 gccagggtgt ggtctccacc cccgtcgcta gcgaccccat cgctctgcag gcaggatacg
      541 acctgctggg cgacggaagg ccagagaccc tgtggctggg aatcggaacc ctgctgatgc
      601 tgatcggcac cttctacttc atcgtgaagg gctggggcgt gaccgacaag gaggccaggg
      661 agtactacag catcacaatc ctggtgcccg gcatcgccag cgccgcctac ctgagcatgt
      721 tcttcggcat cggcctgacc gaggtgaccg tggccggcga ggtgctggac atctactacg
      781 ccagatacgc cgactggctg ttcaccaccc ctttgcttct gctcgacctg gccctgctgg
      841 ctaaggtgga cagggtgagc atcggaaccc tggtgggagt ggacgccctg atgatcgtga
      901 ccggc
//
