LOCUS       sequence-151540-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial vector for cloning and propagation in DH5alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pET3a-OSF-CypA
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..60
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             67..90
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             106..129
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggtggaggtg cccgaggtgg atcgggaggt ggatcgtgga gccacccgca gttcgaaaaa
       61 ggattcgatt acaaggatga cgatgacaag ggtacaagat ctccgctgga agtcctgttc
      121 cagggccccg atatggtcaa ccccaccgtg ttcttcgaca ttgccgtcga cggcgagccc
      181 ttgggccgcg tctcctttga gctgtttgca gacaaggtcc caaagacagc agaaaatttt
      241 cgtgctctga gcactggaga gaaaggattt ggttataagg gttcctgctt tcacagaatt
      301 attccagggt ttatgtgtca gggtggtgac ttcacacgcc ataatggcac tggtggcaag
      361 tccatctatg gggagaaatt tgaagatgag aacttcatcc taaagcatac gggtcctggc
      421 atcttgtcca tggcaaatgc tggacccaac acaaatggtt cccagttttt catctgcact
      481 gccaagactg agtggttgga tggcaagcat gtggtg
//
