LOCUS       sequence-151541-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial vector for cloning and propagation in DH5alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pET3a-OSF-CypA
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(520..543)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(559..582)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(589..612)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(649..672)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             689..711
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(742..761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(743..761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(845..864)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatcctt attcgagttg tccacagtca gcaatggtga tcttcttgct
       61 ggtcttgcca ttcctggacc caaagcgctc catggcctcc acaatattca tgccttcttt
      121 cactttgcca aacaccacat gcttgccatc caaccactca gtcttggcag tgcagatgaa
      181 aaactgggaa ccatttgtgt tgggtccagc atttgccatg gacaagatgc caggacccgt
      241 atgctttagg atgaagttct catcttcaaa tttctcccca tagatggact tgccaccagt
      301 gccattatgg cgtgtgaagt caccaccctg acacataaac cctggaataa ttctgtgaaa
      361 gcaggaaccc ttataaccaa atcctttctc tccagtgctc agagcacgaa aattttctgc
      421 tgtctttggg accttgtctg caaacagctc aaaggagacg cggcccaagg gctcgccgtc
      481 gacggcaatg tcgaagaaca cggtggggtt gaccatatcg gggccctgga acaggacttc
      541 cagcggagat cttgtaccct tgtcatcgtc atccttgtaa tcgaatcctt tttcgaactg
      601 cgggtggctc cacgatccac ctcccgatcc acctcgggca cctccacctt tttcgaactg
      661 cgggtggctc cagctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct
      721 agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc
      781 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      841 ggcgcctata tcgccgacat caccgatggg
//
