LOCUS       sequence-151545-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of mouse EZH2 Y641F mutant, hygromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     pMSCV-Flag-Mm Ezh2 Y641F
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSCV-Flag-Mm Ezh2 Y641F
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatcctg gccaccatgg actacaagga
       61 cgacgatgac aagatgggcc agactgggaa gaaatctgag aagggaccgg tttgttggcg
      121 gaagcgtgta aaatcagagt acatgagact gagacagctc aagaggttca gaagagctga
      181 tgaagtaaag actatgttta gttccaatcg tcagaaaatt ttggaaagaa ctgaaacctt
      241 aaaccaagag tggaagcagc ggaggataca gcctgtgcac atcatgactt ctgtgagctc
      301 attgcgcggg actagggagt gttcagtcac cagtgacttg gattttccag cacaagtcat
      361 cccgttaaag accctgaatg cagtcgcctc ggtgcctata atgtactctt ggtcgccctt
      421 acaacagaat tttatggtgg aagacgaaac tgtcttacat aacattcctt acatggggga
      481 tgaagttctg gatcaggacg gcactttcat tgaagaacta ataaaaaatt atgatggaaa
      541 agtgcatggt gacagagaat gtggatttat aaatgatgaa atttttgtgg agttggtaaa
      601 tgctcttggt caatataatg atgatgatga tgacgatgat ggagatgatc cagatgaaag
      661 agaagaaaaa cagaaagatc tagaggataa tcgagatgat aaagaaactt gcccacctcg
      721 gaaatttcct gctgataaaa tatttgaagc catttcctca atgtttccag ataagggcac
      781 cgcagaagaa ctgaaggaaa aatataaaga actcacggag cagcagctcc caggtgctct
      841 gcctcctgaa tgtactccaa acatcgatgg accaaatgcc aaatctgttc agagggagca
      901 aagcttgcat tcatttcata cgctcttctg tcgacgatgt tttaagtatg actgcttcct
      961 acatcccttc catg
//
