LOCUS       sequence-151547-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial vector for cloning and propagation in DH5alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, 
            Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, 
            Sundquist WI
  TITLE     Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.
  JOURNAL   Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
  PUBMED    27253068
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-06-2"; volume: "5. pii: e16269. doi"; pages: " 
            10.7554/eLife.16269"
COMMENT     Sequence Label: pET11a-CA(A14C, E45C)
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             725..747
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    762..786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(786..805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(787..805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(889..908)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatcctt acaaaactct tgctttatgg ccgggtcccc ccactccctg
       61 acatgctgtc atcatttctt ctagtgtcgc tcctggtccc aatgctttta aaatagtctt
      121 acaatctggg ttcgcatttt ggaccaacaa ggtttctgtc atccaatttt ttacctcttg
      181 tgaagcttgc tcggctctta gagttttata gaatcggtct acatagtctc taaagggttc
      241 ctttggtcct tgtcttatgt ccagaatgct ggtagggcta tacattctta ctattttatt
      301 taatcccagg attatccatc ttttatagat ttctcctact gggataggtg gattatgtgt
      361 catccatcct atttgttcct gaagggtact agtagttcct gctatgtcac ttccccttgg
      421 ttctctcatc tggcctggtg caataggccc tgcatgcact ggatgcaatc tatcccattc
      481 tgcagcttcc tcattgatgg tctcttttaa catttgcatg gctgcttgat gtccccccac
      541 tgtgtttagc atggtattta aatcttgtgg ggtggctccg catgataatg ctgaaaacat
      601 gggtattact tctgggctga aagccttctc ttctactact tttacccatg catttaaagt
      661 tctaggtgat atgcactgat gtaccatttg cccctgcagg ttctgaacga tcggcatatg
      721 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      781 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
      841 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      901 acatcaccga tgg
//
