LOCUS       sequence-151548-         522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  246B. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses 
            rHGH and IFNa2b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP324
FEATURES             Location/Qualifiers
     source          1..522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(418..438)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggccgtcagg gacaaggcca ggctgttctt cttagagcgn ttgatcatga gcgggcttgg
       61 ccaaaggttg gcagctctca tgtctccagc agatggatcc tctagagtcg acgcagctgc
      121 aggtgtgcct ccgccgcata tgttcctcag gtgggttttt aggtgccggt tcaggttgtc
      181 gcggcgactg aagttgcgca tgcatattcg gcattggaat ggcttctcgc cggtgtgcgt
      241 acgtagatgc cccgccaagt gctccttctg ggagaaattt cgcatacaga tccgacactg
      301 aaacggtttt tcaccggtat gagtacgggt atgcctgtca agcctgtcct ggcgcgaaaa
      361 gttccgcatg caaatgcgac actggaaggg gcgctccccg ggtctagatg ccggccggac
      421 tttgcgcttt ttcttaggag ctccgcgcat ttttactagt gtgttttgat agttgttcaa
      481 ttgattgaaa tagggacaaa taaattatct ctatcactga ta
//
