LOCUS       sequence-151549-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  246B. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses 
            rHGH and IFNa2b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP324
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(179..425)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(467..493)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(500..649)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tacatcctca ctccgattga tgtgcggcct cgagaagcag ctgaatcctc actccgattg
       61 atgactggac cgtcaccaat tggagatcct cactccgatt gatggtggta aactcgcaat
      121 atcctcactc cgattgatga attgatctgc cgcgatgact tctatatgat atgctagctc
      181 tcacttaatc ttctgtactc tgaagaggag tgggaaatac caagaaaaac atcaaactcg
      241 aatgattttc ccaaacccct accacaagat attcatcagc tgcgagatag gctgatcagg
      301 agcaagctcg tacgagaaga aacaaaatga caaaaaaaat cctatactat ataggttaca
      361 aataaaaaag tatcaaaaat gaagcctgca tctctcaggc aaatggcatt ctgacatcct
      421 cttgattaga atctagcaag accggtctac cgatggtacc tcaagaagcg tagtccggaa
      481 cgtcgtacgg gtagttaatt aacatatcga gatcgaaatc gtccagagca tcggagccga
      541 gcatgtccag atcaaagtca tccaatgcgt cgcttcccaa catatccagg tcaaagtcat
      601 cgagggcatc agaacccagc atgtcgagat cgaaatcgtc cagcgcgtcg gccctaggcg
      661 atgaagtaga gcccgcagtg gccaagtggc tttggtccgt ctcctccacg gatgcccctc
      721 cacggctagt gggcgcatgt aggcggtggg cgtccagcat ctccagcagc aggtcataga
      781 ggggcaccac gttcttgcac ttcatgctgt acagatgctc catgcctttg ttactcatgt
      841 gcctgatgtg ggagaggatg aggaggagct gggccagccg ctggtgctgc tgctgcaggg
      901 tcaggcctg
//
