LOCUS       sequence-151550-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP positive control (bait) for assay establishment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: PA-RL-BAD
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             158..331
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             332..505
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      121 atccgccgcc accatggttg cgcaacacga tgaagccgtg gacaacaaat tcaacaaaga
      181 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
      241 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
      301 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca
      361 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
      421 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
      481 gctaaatggt gctcaggcgc cgaaagtaga cgcgaattcg atggcttcca aggtgtacga
      541 ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg tgggctcgct gcaagcaaat
      601 gaacgtgctg gactccttca tcaactacta tgattccgag aagcacgccg agaacgccgt
      661 gatttttctg catggtaacg ctgcctccag ctacctgtgg aggcacgtcg tgcctcacat
      721 cgagcccgtg gctagatgca tcatccctga tctgatcgga atgggtaagt ccggcaagag
      781 cgggaatggc tcatatcgcc tcctggatca ctacaagtac ctcaccgctt ggttcgagct
      841 gctgaacctt ccaaagaaaa tcatctttgt gggccacgac tggggggctt gtctggcctt
      901 tcactactcc
//
