LOCUS       sequence-151554-         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  245B. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses 
            rHGH and IFNa2b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP326
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(421..441)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcggccgtc agggacaagg ccaggctgtt cttcttagag cgtttgatca tgagcgggct
       61 tggccaaagg ttggcagctc tcatgtctcc agcagatgga tcctctagag tcgacgcagc
      121 tgcaggtgtg cctccgccgc atatgttcct caggtgggtt tttaggtgcc ggttcaggtt
      181 gtcgcggcga ctgaagttgc gcatgcatat tcggcattgg aatggcttct cgccggtgtg
      241 cgtacgtaga tgccccgcca agtgctcctt ctgggagaaa tttcgcatac agatccgaca
      301 ctgaaacggt ttttcaccgg tatgagtacg ggtatgcctg tcaagcctgt cctggcgcga
      361 aaagttccgc atgcaaatgc gacactggaa ggggcgctcc ccgggtctag atgccggccg
      421 gactttgcgc tttttcttag gagctccgcg catttttact agtgtgtttt gatagttgtt
      481 caattgattg aaatagggac aaataaatta aatttaaagt ctttgggtca ggggaaacca
      541 aaattgggaa aggtgttcgc cttttatatt cgattctggt ggtttccaat aatctcatga
      601 catgcgtccg cccgctat
//
