LOCUS       sequence-151557-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  245B. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses 
            rHGH and IFNa2b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP326
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(133..379)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(421..447)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(454..603)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 cctcactccg attgatgact ggaccgtcac caattggaga tcctcactcc gattgatggt
       61 ggtaaactcg caatatcctc actccgattg atgaattgat ctgccgcgat gacttctata
      121 tgatatgcta gctctcactt aatcttctgt actctgaaga ggagtgggaa ataccaagaa
      181 aaacatcaaa ctcgaatgat tttcccaaac ccctaccaca agatattcat cagctgcgag
      241 ataggctgat caggagcaag ctcgtacgag aagaaacaaa atgacaaaaa aaatcctata
      301 ctatataggt tacaaataaa aaagtatcaa aaatgaagcc tgcatctctc aggcaaatgg
      361 cattctgaca tcctcttgat tagaatctag caagaccggt ctaccgatgg tacctcaaga
      421 agcgtagtcc ggaacgtcgt acgggtagtt aattaacata tcgagatcga aatcgtccag
      481 agcatcggag ccgagcatgt ccagatcaaa gtcatccaat gcgtcgcttc ccaacatatc
      541 caggtcaaag tcatcgaggg catcagaacc cagcatgtcg agatcgaaat cgtccagcgc
      601 gtcggcccta ggcgatgaag tagagcccgc agtggccaag tggctttggt ccgtctcctc
      661 cacggatgcc cctccacggc tagtg
//
