LOCUS       sequence-151561-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP negative control (bait) to exclude unspecific background 
            binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: PA-RL-mCherry
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             157..330
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             331..504
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga
      121 tccgccgcca ccatggttgc gcaacacgat gaagccgtgg acaacaaatt caacaaagaa
      181 caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac
      241 gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct
      301 aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa
      361 aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg aaacgccttc
      421 atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga agctaaaaag
      481 ctaaatggtg ctcaggcgcc gaaagtagac gcgaattcga tggcttccaa ggtgtacgac
      541 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      601 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      661 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      721 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      781 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      841 ctgaaccttc caaagaaaat c
//
