LOCUS       sequence-151566-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP negative control (prey) to exclude unspecific background 
            binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: FL-mCherry
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      129..138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(201..219)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccgccgc caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
      181 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
      241 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
      301 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
      361 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
      421 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
      481 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
      541 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
      601 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
      661 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
      721 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
      781 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
      841 cgattttaag tgttgttcca ttccatcacg gttttggaa
//
