LOCUS       sequence-151568-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP negative control (prey) to exclude unspecific background 
            binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: FL-mCherry
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..45
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(47..754)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(322..341)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     591..609
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(757..781)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(794..835)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tctagagcgg ccgcgatatc accactttgt acaagaaagc tgggtccttg tacagctcgt
       61 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
      121 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
      181 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
      241 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
      301 aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
      361 tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca
      421 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
      481 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
      541 acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct
      601 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
      661 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
      721 tggccatgtt atcctcctcg cccttgctca ccatgaagcc tgcttttttg tacaaacttg
      781 tgatatcctc gagcgtagaa tcgagaccga ggagagggtt agggataggc ttaccgtcga
      841 gccccgcgga attcgcgtcc acggcgatct ttccgccctt cttggccttt atgaggatct
      901 ctctgatttt tcttgcgtcg agttttccgg taagaccttt cggtacttcg tccacaaaca
      961 caactcctcc gcgcaacttt ttcgcggttg ttacttgact gg
//
