LOCUS       sequence-151587-       11211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the WTSI 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 11211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-WTSI
FEATURES             Location/Qualifiers
     source          1..11211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1145..1163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1182..1200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1219..1237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1256..1274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1293..1311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1367..1385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1404..1422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1444..1468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2699..2752
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3728..3781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6622..6646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6688..7261,7262..7264,7265..7273)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6855..6872)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7082..7101
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7274..7984
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7419..7437)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7687..7706
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7996..8015)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8061..8116
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8115..8134)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   8836..8870
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9020..9038)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9059..9075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9059..9075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9072..9094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9083..9099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9107..9113,9114..9131,9132..9137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9152..9173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9290..9307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9461..10049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9541..9560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10220..11011,11012..11080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10843..10862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11081..11185)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aattaactcg agggatccaa gctggccgca
     1141 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     1201 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     1261 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     1321 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     1381 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     1441 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     1501 ccatagaaga caccgggacc gatccagcct ccgcggtggc ggccatcaca agtttgtaca
     1561 aaaaagcagg ctggcgccgg aaccaattca gtcgactgga tccggtaccg aattcagatc
     1621 caccatggct ggacacctgg cttcagactt cgccttctca cccccaccag gtgggggtga
     1681 tgggtcagca gggctggagc cgggctgggt ggattctcga acctggctaa gcttccaagg
     1741 gcctccaggt gggcctggaa tcggaccagg ctcagaggta ttggggatct ccccatgtcc
     1801 gcccgcatac gagttctgcg gagggatggc atactgtgga cctcaggttg gactgggcct
     1861 agtcccccaa gttggcgtgg agactttgca gcctgagggc caggcaggag cacgagtgga
     1921 aagcaactca gagggaacct cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt
     1981 ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg aaagccctgc agaaggagct
     2041 agaacagttt gccaagctgc tgaagcagaa gaggatcacc ttggggtaca cccaggccga
     2101 cgtggggctc accctgggcg ttctctttgg aaaggtgttc agccagacca ccatctgtcg
     2161 cttcgaggcc ttgcagctca gccttaagaa catgtgtaag ctgcggcccc tgctggagaa
     2221 gtgggtggag gaagccgaca acaatgagaa ccttcaggag atatgcaaat cggagaccct
     2281 ggtgcaggcc cggaagagaa agcgaactag cattgagaac cgtgtgaggt ggagtctgga
     2341 gaccatgttt ctgaagtgcc cgaagccctc cctacagcag atcactcaca tcgccaatca
     2401 gcttgggcta gagaaggatg tggttcgagt atggttctgt aaccggcgcc agaagggcaa
     2461 aagatcaagt attgagtatt cccaacgaga agagtatgag gctacaggga cacctttccc
     2521 agggggggct gtatcctttc ctctgccccc aggtccccac tttggcaccc caggctatgg
     2581 aagcccccac ttcaccacac tctactcagt cccttttcct gagggcgagg cctttccctc
     2641 tgttcccgtc actgctctgg gctctcccat gcattcaaac ggcggcgggt ccggaggaga
     2701 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcctggcc caatgtataa
     2761 catgatggag acggagctga agccgccggg cccgcagcaa gcttcggggg gcggcggcgg
     2821 aggaggcaac gccacggcgg cggcgaccgg cggcaaccag aagaacagcc cggaccgcgt
     2881 caagaggccc atgaacgcct tcatggtatg gtcccggggg cagcggcgta agatggccca
     2941 ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgcgg agtggaaact
     3001 tttgtccgag accgagaagc ggccgttcat cgacgaggcc aagcggctgc gcgctctgca
     3061 catgaaggag cacccggatt ataaataccg gccgcggcgg aaaaccaaga cgctcatgaa
     3121 gaaggataag tacacgcttc ccggaggctt gctggccccc ggcgggaaca gcatggcgag
     3181 cggggttggg gtgggcgccg gcctgggtgc gggcgtgaac cagcgcatgg acagctacgc
     3241 gcacatgaac ggctggagca acggcagcta cagcatgatg caggagcagc tgggctaccc
     3301 gcagcacccg ggcctcaacg ctcacggcgc ggcacagatg caaccgatgc accgctacga
     3361 cgtcagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc
     3421 cacctacagc atgtcctact cgcagcaggg cacccccggt atggcgctgg gctccatggg
     3481 ctctgtggtc aagtccgagg ccagctccag cccccccgtg gttacctctt cctcccactc
     3541 cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtacc tccccggcgc
     3601 cgaggtgccg gagcccgctg cgcccagtag actgcacatg gcccagcact accagagcgg
     3661 cccggtgccc ggcacggcca ttaacggcac actgcccctg tcgcacatgg gcggcgggtc
     3721 cggaggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcctggccc
     3781 aatggctgtc agcgacgctc tgctcccgtc cttctccacg ttcgcgtccg gcccggcggg
     3841 aagggagaag acactgcgtc cagcaggtgc cccgactaac cgttggcgtg aggaactctc
     3901 tcacatgaag cgacttcccc cacttcccgg ccgcccctac gacctggcgg cgacggtggc
     3961 cacagacctg gagagtggcg gagctggtgc agcttgcagc agtaacaacc cggccctcct
     4021 agcccggagg gagaccgagg agttcaacga cctcctggac ctagacttta tcctttccaa
     4081 ctcgctaacc caccaggaat cggtggccgc caccgtgacc acctcggcgt cagcttcatc
     4141 ctcgtcttcc ccagcgagca gcggccctgc cagcgcgccc tccacctgca gcttcagcta
     4201 tccgatccgg gccgggggtg acccgggcgt ggctgccagc aacacaggtg gagggctcct
     4261 ctacagccga gaatctgcgc cacctcccac ggcccccttc aacctggcgg acatcaatga
     4321 cgtgagcccc tcgggcggct tcgtggctga gctcctgcgg ccggagttgg acccagtata
     4381 cattccgcca cagcagcctc agccgccagg tggcgggctg atgggcaagt ttgtgctgaa
     4441 ggcgtctctg accacccctg gcagcgagta cagcagccct tcggtcatca gtgttagcaa
     4501 aggaagccca gacggcagcc accccgtggt agtggcgccc tacagcggtg gcccgccgcg
     4561 catgtgcccc aagattaagc aagaggcggt cccgtcctgc acggtcagcc ggtccctaga
     4621 ggcccatttg agcgctggac cccagctcag caacggccac cggcccaaca cacacgactt
     4681 ccccctgggg cggcagctcc ccaccaggac tacccctaca ctgagtcccg aggaactgct
     4741 gaacagcagg gactgtcacc ctggcctgcc tcttccccca ggattccatc cccatccggg
     4801 gcccaactac cctcctttcc tgccagacca gatgcagtca caagtcccct ctctccatta
     4861 tcaagagctc atgccaccgg gttcctgcct gccagaggag cccaagccaa agaggggaag
     4921 aaggtcgtgg ccccggaaaa gaacagccac ccacacttgt gactatgcag gctgtggcaa
     4981 aacctatacc aagagttctc atctcaaggc acacctgcga actcacacag gcgagaaacc
     5041 ttaccactgt gactgggacg gctgtgggtg gaaattcgcc cgctccgatg aactgaccag
     5101 gcactaccgc aaacacacag ggcaccggcc ctttcagtgc cagaagtgcg acagggcctt
     5161 ttccaggtcg gaccaccttg ccttacacat gaagaggcac tttggcagcg gaaaacagct
     5221 gttgaatttt gaccttctca agttggcggg agacgtggag tccaacccag ggcccgtcga
     5281 gatgcccctc aacgtgaact tcaccaacag gaactatgac ctcgactacg actccgtaca
     5341 gccctatttc atctgcgacg aggaagagaa tttctatcac cagcaacagc agagcgagct
     5401 gcagccgccc gcgcccagtg aggatatctg gaagaaattc gagctgcttc ccaccccgcc
     5461 cctgtccccg agccgccgct ccgggctctg ctctccatcc tatgttgcgg tcgctacgtc
     5521 cttctcccca agggaagacg atgacggcgg cggtggcaac ttctccaccg ccgatcagct
     5581 ggagatgatg accgagttac ttggaggaga catggtgaac cagagcttca tctgcgatcc
     5641 tgacgacgag accttcatca agaacatcat catccaggac tgtatgtgga gcggtttctc
     5701 agccgctgcc aagctggtct cggagaagct ggcctcctac caggctgcgc gcaaagacag
     5761 caccagcctg agccccgccc gcgggcacag cgtctgctcc acctccagcc tgtacctgca
     5821 ggacctcacc gccgccgcgt ccgagtgcat tgacccctca gtggtctttc cctacccgct
     5881 caacgacagc agctcgccca aatcctgtac ctcgtccgat tccacggcct tctctccttc
     5941 ctcggactcg ctgctgtcct ccgagtcctc cccacgggcc agccctgagc ccctagtgct
     6001 gcatgaggag acaccgccca ccaccagcag cgactctgaa gaagagcaag aagatgagga
     6061 agaaattgat gtggtgtctg tggagaagag gcaaacccct gccaagaggt cggagtcggg
     6121 ctcatctcca tcccgaggcc acagcaaacc tccgcacagc ccactggtcc tcaagaggtg
     6181 ccacgtctcc actcaccagc acaactacgc cgcacccccc tccacaagga aggactatcc
     6241 agctgccaag agggccaagt tggacagtgg cagggtcctg aagcagatca gcaacaaccg
     6301 caagtgctcc agccccaggt cctcagacac ggaggaaaac gacaagaggc ggacacacaa
     6361 cgtcttggaa cgtcagagga ggaacgagct gaagcgcagc ttttttgccc tgcgtgacca
     6421 gatccctgaa ttggaaaaca acgaaaaggc ccccaaggta gtgatcctca aaaaagccac
     6481 cgcctacatc ctgtccattc aagcagacga gcacaagctc acctctgaaa aggacttatt
     6541 gaggaaacga cgagaacagt tgaaacacaa actcgaacag cttcgaaact ctggtgcatg
     6601 agtcgagatg catcgatcta gacccagctt tcttgtacaa agtggtgatc tagctaaaac
     6661 gcggcctcga atcgaattcc gccccccccc ctctccctcc ccccccccta acgttactgg
     6721 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     6781 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     6841 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     6901 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     6961 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     7021 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     7081 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     7141 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     7201 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     7261 tatggccaca accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     7321 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     7381 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     7441 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     7501 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     7561 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     7621 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     7681 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     7741 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     7801 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     7861 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     7921 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     7981 gtaaagcggc cgcgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     8041 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     8101 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     8161 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt
     8221 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     8281 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     8341 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     8401 tactcccagt catagctgtc cctcttctct tatgaagatc cctcgacctg caggaattcg
     8461 atatcaagct ctagccaaca agctcgtcat cgctttgcag aagagcagag aggatatgct
     8521 catcgtctaa agaactaccc attttattat atattagtca cgatatctat aacaagaaaa
     8581 tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg
     8641 agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt
     8701 tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag
     8761 cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc
     8821 aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaaaaaaga
     8881 tttgcgcttt actcgaccta aactttaaac acgtcataga atcttcgttt gacaaaaacc
     8941 acattgtggc caagctgtgt gacgcgacgc gcgctaaaga atggcaaacc aagtcgcgcg
     9001 aggtacccag cttttgttcc ctttagtgag ggttaattcc gagcttggcg taatcatggt
     9061 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9121 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9241 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9301 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9361 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9421 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9481 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9541 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9601 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9661 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9721 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9781 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9841 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9901 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9961 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10021 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10081 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10141 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10201 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10261 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10321 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10381 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10441 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10501 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10561 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10621 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10681 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10741 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10801 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10861 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10921 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10981 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11041 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11101 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11161 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
