LOCUS       sequence-151634-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Assays activity of transcriptional activators fused to ST1 Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: gRNA_tdtomato_reporter_1_ST1_2x_binding
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        156..194
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     191..215
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      229..238
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             238..261
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(419..437)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     687..706
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggcgaattgg gccctctaga tgcatgctcg aggtcccctc caccccacag tgcaagaaac
       61 ggccgccagt gtgatggata tctgcagaat tcgcccttgc gacgtaggga taacagggta
      121 atagtgtccc ctccacccca cagtgcaaga aacgaggtag gcgtgtacgg tgggaggcct
      181 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcgc caccatggac
      241 tacaaggatg acgacgataa aacttccggt ggcggactgg gttccaccgt gagcaagggc
      301 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
      361 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      421 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      481 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
      541 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
      601 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
      661 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      721 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
//
