LOCUS       sequence-151635-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Assays activity of transcriptional activators fused to ST1 Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: gRNA_tdtomato_reporter_1_ST1_2x_binding
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     142..159
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    170..251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     195..214
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(534..557)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(732..751)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg
       61 ccgccagtgt gctggaattc gccctttatt accgcctttg agtgagctga taccgctcgc
      121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagctcgagg cagtgaaaaa
      181 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      241 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
      301 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
      361 tagagatatc gtcgacaagc ttatcgatgc ggccgcgcta gcacgcgtca gctgactaga
      421 ggatccccgg gtaccgagct cgaattctta cttgtacagc tcgtccatgc cgtacaggaa
      481 caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg
      541 ggaggtgatg tccagcttgg tgtccacgta gtagtagccg ggcagttgca cgggcttctt
      601 ggccatgtag atggtcttga actccaccag gtagtggccg ccgtccttca gcttcagggc
      661 ctggtggatc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc
      721 ccagcccatg gtcttcttct gcattacggg gccgtcgggg gggaagttgg tgccgcgcat
      781 cttcaccttg tagatcagcg tgcc
//
