LOCUS       sequence-151637-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Assays activity of transcriptional activators fused to SA Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: gRNA_tdtomato_reporter_1_SA-2x
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        155..193
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     190..214
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      228..237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             237..260
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(418..436)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 attgggccct ctagatgcat gctcgagacc agtcccctcc accccacagt gaagaatcgg
       61 ccgccagtgt gatggatatc tgcagaattc gccctttagg gataacaggg taatagtacc
      121 agtcccctcc accccacagt gaagaatggt gcgaggtagg cgtgtacggt gggaggccta
      181 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agaattcgcc accatggact
      241 acaaggatga cgacgataaa acttccggtg gcggactggg ttccaccgtg agcaagggcg
      301 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
      361 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      421 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
      481 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
      541 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
      601 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
      661 gcggcaccaa cttccccccc gacggccccg taa
//
