LOCUS       sequence-151638-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Assays activity of transcriptional activators fused to SA Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: gRNA_tdtomato_reporter_1_SA-2x
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     135..152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    163..244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     188..207
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(527..550)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(725..744)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atactcaagc tatgcatcaa gcttggtacc gagctcggat ccactagtaa cggccgccag
       61 tgtgctggaa ttcgcccttt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      121 gaacgaccga gcgcagcgag tcagtgagcg aggaagctcg aggcagtgaa aaaaatgctt
      181 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
      241 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
      301 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagagat
      361 atcgtcgaca agcttatcga tgcggccgcg ctagcacgcg tcagctgact agaggatccc
      421 cgggtaccga gctcgaattc ttacttgtac agctcgtcca tgccgtacag gaacaggtgg
      481 tggcggccct cggagcgctc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
      541 atgtccagct tggtgtccac gtagtagtag ccgggcagtt gcacgggctt cttggccatg
      601 tagatggtct tgaactccac caggtagtgg ccgccgtcct tcagcttcag ggcctggtgg
      661 atctcgccct tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc ctcccagccc
      721 atggtcttct tctgcattac ggggccgtcg gg
//
