LOCUS       sequence-151639-        9221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of mouse EZH2 Y641F mutant, hygromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9221)
  TITLE     pMSCV-Flag-Mm Ezh2 Y641F
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSCV-Flag-Mm Ezh2 Y641F
FEATURES             Location/Qualifiers
     source          1..9221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1418..1427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1427..1450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        3714..4213
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3749..3772)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4146..4165
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4231..5256
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4231..5256
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             5614..6128
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6290..6306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6314..6320,6321..6338,6339..6344))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6359..6380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6497..6514)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6668..7256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6748..6767)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7427..8218,8219..8287))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8050..8069
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8288..8392)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8460..8478
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8516..8538)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8638..8657
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8851..8873
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9001..9020
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9220..9221,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatcctggcc accatggact acaaggacga
     1441 cgatgacaag atgggccaga ctgggaagaa atctgagaag ggaccggttt gttggcggaa
     1501 gcgtgtaaaa tcagagtaca tgagactgag acagctcaag aggttcagaa gagctgatga
     1561 agtaaagact atgtttagtt ccaatcgtca gaaaattttg gaaagaactg aaaccttaaa
     1621 ccaagagtgg aagcagcgga ggatacagcc tgtgcacatc atgacttctg tgagctcatt
     1681 gcgcgggact agggagtgtt cagtcaccag tgacttggat tttccagcac aagtcatccc
     1741 gttaaagacc ctgaatgcag tcgcctcggt gcctataatg tactcttggt cgcccttaca
     1801 acagaatttt atggtggaag acgaaactgt cttacataac attccttaca tgggggatga
     1861 agttctggat caggacggca ctttcattga agaactaata aaaaattatg atggaaaagt
     1921 gcatggtgac agagaatgtg gatttataaa tgatgaaatt tttgtggagt tggtaaatgc
     1981 tcttggtcaa tataatgatg atgatgatga cgatgatgga gatgatccag atgaaagaga
     2041 agaaaaacag aaagatctag aggataatcg agatgataaa gaaacttgcc cacctcggaa
     2101 atttcctgct gataaaatat ttgaagccat ttcctcaatg tttccagata agggcaccgc
     2161 agaagaactg aaggaaaaat ataaagaact cacggagcag cagctcccag gtgctctgcc
     2221 tcctgaatgt actccaaaca tcgatggacc aaatgccaaa tctgttcaga gggagcaaag
     2281 cttgcattca tttcatacgc tcttctgtcg acgatgtttt aagtatgact gcttcctaca
     2341 tcccttccat gcaacaccca acacatataa gaggaagaac acagaaacag ctttggacaa
     2401 caagccttgt ggaccacagt gttaccagca tctggaggga gctaaggagt ttgctgctgc
     2461 tcttactgct gagcggataa agacaccacc taaacgccca gggggccgca gaagaggaag
     2521 acttccgaat aacagtagca gacccagcac ccccaccatc agtgtgctgg agtcaaagga
     2581 tacagacagt gacagagaag cagggactga aactggggga gagaacaacg ataaagaaga
     2641 agaagagaaa aaagatgaga cgtccagctc ctctgaagca aattctcggt gtcaaacacc
     2701 aataaagatg aagccaaata ttgaacctcc tgagaatgtg gagtggagtg gtgctgaagc
     2761 ctccatgttt agagtcctca ttggtactta ctacgataac ttttgtgcca ttgctaggct
     2821 aattgggacc aaaacatgta gacaggtgta tgagtttaga gtcaaggagt ccagtatcat
     2881 agcacctgtt cccactgagg atgtagacac tcctccaaga aagaagaaaa ggaaacatcg
     2941 gttgtgggct gcacactgca gaaagataca actgaaaaag gacggctcct ctaaccatgt
     3001 ttacaactat caaccctgtg accatccacg gcagccttgt gacagttcgt gcccttgtgt
     3061 gatagcacaa aatttttgtg aaaagttttg tcaatgtagt tcagagtgtc aaaaccgctt
     3121 tcctggatgt cggtgcaaag cacaatgcaa caccaaacag tgtccatgct acctggctgt
     3181 ccgagagtgt gaccctgacc tctgtctcac gtgtggagct gctgaccatt gggacagtaa
     3241 aaatgtatcc tgtaagaact gtagcattca gcggggctct aaaaagcact tactgctggc
     3301 accgtctgat gtggcaggct ggggcatctt tatcaaagat cctgtacaaa aaaatgaatt
     3361 catctcagaa ttctgtgggg agattatttc tcaggatgaa gcagacagaa gaggaaaagt
     3421 gtatgacaaa tacatgtgca gctttctgtt caacttgaac aatgattttg tggtggatgc
     3481 aacccgaaag ggcaacaaaa ttcgttttgc taatcattca gtaaatccaa actgctatgc
     3541 aaaagttatg atggttaatg gtgaccacag gataggcatc tttgctaaga gggctatcca
     3601 gactggtgaa gagttgtttt ttgattacag atacagccag gctgatgccc tgaagtatgt
     3661 gggcatcgaa cgagaaatgg aaatcccttg actcgaggtt aacgaattct accgggtagg
     3721 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     3781 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     3841 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     3901 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     3961 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     4021 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     4081 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     4141 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     4201 tccgggcctt tcgacctgca tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct
     4261 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     4321 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     4381 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     4441 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     4501 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     4561 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     4621 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     4681 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     4741 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     4801 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     4861 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     4921 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     4981 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     5041 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     5101 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     5161 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     5221 cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc
     5281 tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga
     5341 acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg
     5401 tcgcgggagg gccggtcgca gtgattcagg cccttctgga ttgtgttggt ccccagggca
     5461 cgattgtcat gcccacgcac tcgggtgatc tgactgatcc cgcagattgg agatcgccgc
     5521 ccgtgcctgc cgattgggtg cagatccgtc gacctgcagc caagcttatc gataaaataa
     5581 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     5641 aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga
     5701 agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt
     5761 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg
     5821 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     5881 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     5941 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     6001 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     6061 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     6121 tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa
     6181 tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc aatactcctg
     6241 aaatagttca ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc
     6301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6421 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6481 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6601 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6661 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6721 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6781 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6841 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6901 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6961 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7021 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7081 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7141 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7201 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7261 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7321 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7381 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7441 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7501 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7561 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7621 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7681 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7741 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7801 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7861 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7921 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7981 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8041 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8101 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8161 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8221 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8281 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8341 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8401 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8461 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     8521 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     8581 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     8641 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     8701 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     8761 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     8821 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcgc
     8881 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     8941 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     9001 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     9061 gatgcgtccg gcgtagaggc gattagtcca atttgttaaa gacaggatat cagtggtcca
     9121 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
     9181 aaaataaaag attttattta gtctccagaa aaagggggga a
//
