LOCUS       sequence-151641-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruits VP64 to a compatible gRNA for transcriptional activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pcDNA3_3_TOPO_TA-ms2-VP64
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             57..77
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             87..110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             492..641
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(708..727)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 accgggaccg atccagcctc cggactctag aggatcgaac ccttgccacc atgggaccta
       61 agaaaaagag gaaggtggcg gccgctgact acaaggatga cgacgataaa tctagaatgg
      121 cttctaactt tactcagttc gttctcgtcg acaatggcgg aactggcgac gtgactgtcg
      181 ccccaagcaa cttcgctaac gggatcgctg aatggatcag ctctaactcg cgttcacagg
      241 cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa tacaccatca
      301 aagtcgaggt gcctaaaggc gcctggcgtt cgtacttaaa tatggaacta accattccaa
      361 ttttcgccac gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag
      421 atggaaaccc gattccctca gcaatcgcag caaactccgg catctacgag gccagcggtt
      481 ccggacgggc tgacgcattg gacgattttg atctggatat gctgggaagt gacgccctcg
      541 atgattttga ccttgacatg cttggttcgg atgcccttga tgactttgac ctcgacatgc
      601 tcggcagtga cgcccttgat gatttcgacc tggacatgct gattaactct agatgaaagg
      661 gttcgatccc taccggttag taatgagttt aaacggggga ggctaactga aacacggaag
      721 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
      781 gg
//
