LOCUS       sequence-151643-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruits VP64 to a compatible gRNA for transcriptional activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pcDNA3_3_TOPO_TA-ms2-VP64
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    46..94
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     119..138
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(205..354)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(736..759)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(769..789)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      792..801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
       61 cacccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt
      121 gtctccttcc gtgtttcagt tagcctcccc cgtttaaact cattactaac cggtagggat
      181 cgaacccttt catctagagt taatcagcat gtccaggtcg aaatcatcaa gggcgtcact
      241 gccgagcatg tcgaggtcaa agtcatcaag ggcatccgaa ccaagcatgt caaggtcaaa
      301 atcatcgagg gcgtcacttc ccagcatatc cagatcaaaa tcgtccaatg cgtcagcccg
      361 tccggaaccg ctggcctcgt agatgccgga gtttgctgcg attgctgagg gaatcgggtt
      421 tccatctttt aggagacctt gcattgcctt aacaataagc tcgcagtcgg aattcgtggc
      481 gaaaattgga atggttagtt ccatatttaa gtacgaacgc caggcgcctt taggcacctc
      541 gactttgatg gtgtatttgc gattctgcgc agagctctga cgaacgctac aggttacttt
      601 gtaagcctgt gaacgcgagt tagagctgat ccattcagcg atcccgttag cgaagttgct
      661 tggggcgaca gtcacgtcgc cagttccgcc attgtcgacg agaacgaact gagtaaagtt
      721 agaagccatt ctagatttat cgtcgtcatc cttgtagtca gcggccgcca ccttcctctt
      781 tttcttaggt cccatggtgg caagg
//
