LOCUS       sequence-151651-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruits p65_hsf1 to compatible gRNAs for transcriptional 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pLEX_307_ms2_p65_hsf1
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..53
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             62..448
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             503..523
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgtcgtgag gctagcatcg attgatcaac aagtttgtac aaaaaagcag gctacaaaat
       61 ggcttcaaac tttactcagt tcgtgctcgt ggacaatggt gggacagggg atgtgacagt
      121 ggctccttct aatttcgcta atggggtggc agagtggatc agctccaact cacggagcca
      181 ggcctacaag gtgacatgca gcgtcaggca gtctagtgcc cagaagagaa agtataccat
      241 caaggtggag gtccccaaag tggctaccca gacagtgggc ggagtcgaac tgcctgtcgc
      301 cgcttggagg tcctacctga acatggagct cactatccca attttcgcta ccaattctga
      361 ctgtgaactc atcgtgaagg caatgcaggg gctcctcaaa gacggtaatc ctatcccttc
      421 cgccatcgcc gctaactcag gtatctacag cgctggagga ggtggaagcg gaggaggagg
      481 aagcggagga ggaggtagcg gacctaagaa aaagaggaag gtggcggccg ctggatcccc
      541 ttcagggcag atcagcaacc aggccctggc tctggcccct agctccgctc cagtgctggc
      601 ccagactatg gtgccctcta gtgctatggt gcctctggcc cagccacctg ctccagcccc
      661 tgtgctgacc ccaggaccac cccagtcact gagcgctcca gtgcccaagt ctacacaggc
      721 cggcgagggg actctgagtg aagctctgct gcacctgcag ttcgacgctg atgaggacct
      781 gggagctctg ctgg
//
