LOCUS       sequence-151652-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruits VP64 to compatible gRNAs for transcriptional activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pLEX_307_ms2_VP64
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             56..76
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             86..109
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             491..640
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     protein_bind    complement(657..681)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             689..730
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggctagcatc gattgatcaa caagtttgta caaaaaagca ggctacaaaa tgggacctaa
       61 gaaaaagagg aaggtggcgg ccgctgacta caaggatgac gacgataaat ctagaatggc
      121 ttctaacttt actcagttcg ttctcgtcga caatggcgga actggcgacg tgactgtcgc
      181 cccaagcaac ttcgctaacg ggatcgctga atggatcagc tctaactcgc gttcacaggc
      241 ttacaaagta acctgtagcg ttcgtcagag ctctgcgcag aatcgcaaat acaccatcaa
      301 agtcgaggtg cctaaaggcg cctggcgttc gtacttaaat atggaactaa ccattccaat
      361 tttcgccacg aattccgact gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga
      421 tggaaacccg attccctcag caatcgcagc aaactccggc atctacgagg ccagcggttc
      481 cggacgggct gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga
      541 tgattttgac cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct
      601 cggcagtgac gcccttgatg atttcgacct ggacatgctg attaactcta gatgaaaccc
      661 agctttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
      721 cgattctacg tagtaatgaa ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac
      781 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
      841 cctt
//
