LOCUS       sequence-151653-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruits VP64 to compatible gRNAs for transcriptional activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pLEX_307_ms2_VP64
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(76..117)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(166..315)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 aaattttgta atccagaggt tgattgtcga cttaacgcgt tgattgtcga cttaaccggt
       61 actagttcat tactacgtag aatcgagacc gaggagaggg ttagggatag gcttaccgat
      121 atcaaccact ttgtacaaga aagctgggtt tcatctagag ttaatcagca tgtccaggtc
      181 gaaatcatca agggcgtcac tgccgagcat gtcgaggtca aagtcatcaa gggcatccga
      241 accaagcatg tcaaggtcaa aatcatcgag ggcgtcactt cccagcatat ccagatcaaa
      301 atcgtccaat gcgtcagccc gtccggaacc gctggcctcg tagatgccgg agtttgctgc
      361 gattgctgag ggaatcgggt ttccatcttt taggagacct tgcattgcct taacaataag
      421 ctcgcagtcg gaattcgtgg cgaaaattgg aatggttagt tccatattta agtacgaacg
      481 ccaggcgcct ttaggcacct cgactttgat ggtgtatttg cgattctgcg cagagctctg
      541 acgaacgcta caggttactt tgtaagcctg tgaacgcgag ttagagctga tccattcagc
      601 gatcccgtta gcgaagttgc ttggg
//
