LOCUS       sequence-151656-         544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruits VPR to compatible gRNAs for transcriptional activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pLEX_307_ms2_VPR
FEATURES             Location/Qualifiers
     source          1..544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..46
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaagaggaag gtggcggccg ctgactacaa ggatgacgac gataaatcta gaatggcttc
       61 taactttact cagttcgttc tcgtcgacaa tggcggaact ggcgacgtga ctgtcgcccc
      121 aagcaacttc gctaacggga tcgctgaatg gatcagctct aactcgcgtt cacaggctta
      181 caaagtaacc tgtagcgttc gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt
      241 cgaggtgcct aaaggcgcct ggcgttcgta cttaaatatg gaactaacca ttccaatttt
      301 cgccacgaat tccgactgcg agcttattgt taaggcaatg caaggtctcc taaaagatgg
      361 aaacccgatt ccctcagcaa tcgcagcaaa ctccggcatc tacgaggcca gcgaggccag
      421 cggttccgga cgggctgacg cattggacga ttttgatctg gatatgctgg gaagtgacgc
      481 cctcgatgat tttgaccttg acatgcttgg ttcggatgcc cttgatgact ttgacctcga
      541 catg
//
