LOCUS       sequence-151664-         349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 gRNA variant to recruit ms2 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chim...
FEATURES             Location/Qualifiers
     source          1..349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     119..138
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        275..293
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        330..348
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
       61 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      121 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      181 gaaaggacga aacaccggag acgattaatg cgtctcggtt ttagagctag aaatagcaag
      241 ttaaaataag gctagtccgt tatcaacttg gccaacatga ggatcaccca tgtctgcagg
      301 gccaagtggc accgagtcgg tgcgggagca catgaggatc acccatgtg
//
