LOCUS       sequence-151679-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of mature phosphate binding 
            protein, PstS, as scaffold for phosphate biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Solscheid C, Kunzelmann S, Davis CT, Hunter JL, Nofer A, Webb MR
  TITLE     Development of a Reagentless Biosensor for Inorganic Phosphate, 
            Applicable over a Wide Concentration Range.
  JOURNAL   Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 
            10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
  PUBMED    26199994
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.5b00449"; journalName: "Biochemistry"; date: 
            "2015-08-18- 18"; volume: "54"; issue: "32"; pages: "5054-62"
COMMENT     Sequence Label: pET22b_PstS_2
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1038..1060
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    1075..1099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtc
       61 attagtacag cggcttaccg ctactgtctt taatattggt cttccacgca gcgcgaacct
      121 gttcaactac actatccggc aggctggcgt aatccaggtc gttcgcctgt ttagccccgg
      181 ttttgtacgc ccagtcgaag aatttcagca cttctgtgcc ttgttctggt ttcttctgat
      241 ctttgtggat cagaatgaac gtggtagagg taataggcca tgcatcttcg cctttctggt
      301 tggtcagatc ctgagcgaag gttttgctcc agtctgcacc ttttgctgca ttagcgaagt
      361 tttcttcggt cggactaacc ggtttaccat cagcggagat cagtttggtg tacgccaggt
      421 tgttctgctt cgcgtaacaa tattcaacat aaccaattgc acccggcaga cgctgaacga
      481 acgcggcgat accgtcgtta cctttaccgc ccagaccgat cggccatttt acggtagagc
      541 cagtaccaac gttgtttttc cactcttcgt tcactttcgc caggtagctg gtgaagacga
      601 aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttttgtgaa ggcagtttca
      661 gacccggatt cagtttggcg atggcttcat catcccactt cttgattttg cccaggtaga
      721 tgtcgccgag ggttttacca tccagcacca gttcgccaga cttcagccct ggaatgttaa
      781 ccgccagcac cacgccgcca atcacggtcg ggaactggaa cagaccttcc tgagccagtt
      841 tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac ggtattagcg ataatctgtt
      901 ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt tctttctggt
      961 aagtgtcagc ccatttgcaa tacaccggcg cagggaaggt tgcacctgca cctgtcaggc
     1021 ttgcttccat atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt
     1081 gttatccgct cacaattccc ctatag
//
