LOCUS       sequence-151685-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCS-containing binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB200
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..602
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg cagatctgtc
       61 gatcgaccat ggtacgcagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
      121 agttaggtgg ctcaagtatg ggcatcattc gcacatgtag gctcggccct gaccaagtca
      181 aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt cggagacgta gccacctact
      241 cccaacatca gccggactcc gattacctcg ggaacttgct ccgtagtaag acattcatcg
      301 cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct cgcggcttac gttctgccca
      361 agtttgagca gccgcgtagt gagatctata tctatgatct cgcagtctcc ggcgagcacc
      421 ggaggcaggg cattgccacc gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg
      481 gtgcttatgt gatctacgtg caagcagatt acggtgacga tcccgcagtg gctctctata
      541 caaagttggg catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct
      601 aacaattcgt tcaagccgag atcggcttcc cggcctagag tcgatcgaca agctcgagtt
      661 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
      721 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aa
//
