LOCUS       sequence-151688-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..743
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcgagc
       61 tgattaagga gaacatgccc atgaagctgt acatggaggg caccgtgaac aaccaccact
      121 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
      181 aggtcgtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
      241 acggcagcag aaccttcatc aagcaccctc cgggcatccc cgacttcttt aagcagtcct
      301 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
      361 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag gttagagggg
      421 tgaacttccc agccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca
      481 ccgagacgat gtaccccgct gacggcggcc tggaaggcgc atgtgacatg gccctgaagc
      541 tcgtgggcgg gggccacctg atctgcaacc ttgagaccac atacagatcc aagaaacccg
      601 ctacgaacct caagatgccc ggcgtctaca acgtggacca cagactggaa agaatcaagg
      661 aggccgacga tgagacctac gtcgagcagc acgaggtggc tgtggccaga tactctactg
      721 gtggcgctgg tgatggaggt aaaggtggag gaggttccgg actcagatct ggcagcggtg
      781 gaggcttgga ggagccggag gccgcgacgc ggacagccgc ggccgtggat tgcaaagaca
      841 gacccgggtt cccagtgaag aggttaatac aagcccgctt gcccttcaaa cgcctgaacc
      901 ttgttc
//
