LOCUS       sequence-151689-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of mature phosphate binding 
            protein, PstS, which also has a mutation to weaken phosphate 
            binding, as scaffold for phosphate biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Solscheid C, Kunzelmann S, Davis CT, Hunter JL, Nofer A, Webb MR
  TITLE     Development of a Reagentless Biosensor for Inorganic Phosphate, 
            Applicable over a Wide Concentration Range.
  JOURNAL   Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 
            10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
  PUBMED    26199994
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.5b00449"; journalName: "Biochemistry"; date: 
            "2015-08-18- 18"; volume: "54"; issue: "32"; pages: "5054-62"
COMMENT     Sequence Label: pET22b_PstS_3
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatggaagc aagcctgaca
       61 ggtgcaggtg caaccttccc tgcgccggtg tattgcaaat gggctgacac ttaccagaaa
      121 gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt
      181 atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga aaaactggct
      241 caggaaggtc tgttccagtt cccgaccgtg ggtggcggcg tggtgctggc ggttaacatt
      301 ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga catctacctg
      361 ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg tctgaaactg
      421 ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc cttcgtcttc
      481 accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac tggctctacc
      541 gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc gttcgttcag
      601 cgtctgccgg gtgcaattgg ttatgttgaa tattgttacg cgaagcagaa caacctggcg
      661 tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga aaacttcgct
      721 aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac caaccagaaa
      781 ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa agatcagaag
      841 aaaccagaac aaggcacaga agtgctgaaa ttcttcgact gggcgtacaa aaccggggct
      901 aaacaggcga acgacctgga ttacgc
//
