LOCUS       sequence-151690-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of mature phosphate binding 
            protein, PstS, which also has a mutation to weaken phosphate 
            binding, as scaffold for phosphate biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Solscheid C, Kunzelmann S, Davis CT, Hunter JL, Nofer A, Webb MR
  TITLE     Development of a Reagentless Biosensor for Inorganic Phosphate, 
            Applicable over a Wide Concentration Range.
  JOURNAL   Biochemistry. 2015 Aug 18;54(32):5054-62. doi: 
            10.1021/acs.biochem.5b00449. Epub 2015 Aug 4.
  PUBMED    26199994
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.5b00449"; journalName: "Biochemistry"; date: 
            "2015-08-18- 18"; volume: "54"; issue: "32"; pages: "5054-62"
COMMENT     Sequence Label: pET22b_PstS_3
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1037..1059
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtca
       61 ttagtacagc ggcttaccgc tactgtcttt aatattggtc ttccacgcag cgcgaacctg
      121 ttcaactaca ctatccggca ggctggcgta atccaggtcg ttcgcctgtt tagccccggt
      181 tttgtacgcc cagtcgaaga atttcagcac ttctgtgcct tgttctggtt tcttctgatc
      241 tttgtggatc agaatgaacg tggtagaggt aataggccat gcatcttcgc ctttctggtt
      301 ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct tttgctgcat tagcgaagtt
      361 ttcttcggtc ggactaaccg gtttaccatc agcggagatc agtttggtgt acgccaggtt
      421 gttctgcttc gcgtaacaat attcaacata accaattgca cccggcagac gctgaacgaa
      481 cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc ggccatttta cggtagagcc
      541 agtaccaacg ttgtttttcc actcttcgtt cactttcgcc aggtagctgg tgaagacgaa
      601 ggaagtcccg gagccatctg cgcggcgtac tacagcaatg ttttgtgaag gcagtttcag
      661 acccggattc agtttggcga tggcttcatc atcccacttc ttgattttgc ccaggtagat
      721 gtcgccgagg gttttaccat ccagcaccag ttcgccagac ttcagccctg gaatgttaac
      781 cgccagcacc acgccgccac ccacggtcgg gaactggaac agaccttcct gagccagttt
      841 ttcgtcagac agcggcgcgt cagaggcacc aaaatcaacg gtattagcga taatctgttt
      901 tacgccaccg gaagaaccga taccctggta gttaacttta ttaccggttt ctttctggta
      961 agtgtcagcc catttgcaat acaccggcgc agggaaggtt gcacctgcac ctgtcaggct
     1021 tgcttccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg
     1081 ttatcc
//
