LOCUS       sequence-151695-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of single-cysteine variant of plasmid segregation 
            protein, ParM, as scaffold for ADP biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Kunzelmann S, Webb MR
  TITLE     A biosensor for fluorescent determination of ADP with high time 
            resolution.
  JOURNAL   J Biol Chem. 2009 Nov 27;284(48):33130-8. doi: 
            10.1074/jbc.M109.047118. Epub 2009 Sep 29.
  PUBMED    19801632
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.047118"; journalName: "J Biol Chem"; date: 
            "2009-11-27- 27"; volume: "284"; issue: "48"; pages: "33130-8"
COMMENT     Sequence Label: pHis17_ParM _1
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(63..80)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(90..107)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat cgttaatgat gatgatgatg atggaattct
       61 tagtggtggt ggtggtggtg aagcttttaa tgatgatgat gatgatggga tcctgattga
      121 ttacctatga gatacatacc gttaactaaa tcatattgag agttattggt tttgaaaaaa
      181 cgttcatcac gaatctgtgt gtgttttttt actgcatcgg ctattaattc tgcgccaccg
      241 cctataacca taacatgagt ataaccagaa aattcattga gcgtatttaa tacacgttgc
      301 tcaagtttac gaagtgcttc attcattgct tcggtgacta ttgatatttt gttctcatca
      361 ttaattcgtt gcttcagata gttattatct tttctgtgaa tgattatatc gtcagcaaga
      421 tagctacttc cttttgttct cgcaagagaa agggcatctt ttactgcaga tgtaaccaga
      481 gagacaccaa gagatgagtc tccgtatatt ttactgatcc ccgataattt ccccattacc
      541 tgagaaatat ctaagttggc gcccccgaga tctataatta ataaagaatc taactcatcc
      601 agttcttgta gaacttcata acctgccggt atagattcag gcatgacttt tacatctttt
      661 attgtgaatg tatccccgcc atttaatgta atttttttcc ggaagtttgc tttcttacgc
      721 tcaatatttt ccgtattggg ttggttattt ctgtcgtaat actctgtcag aggaagtgtg
      781 caaacaatat ccacttcgct taccggcaga ccactggtca gtaaggcgtg atgcactgca
      841 acgacattaa cgtcgctgta ttgccatgcg atattggttg tgactacagc atccgggctg
      901 attggatcaa atgaatactg ttcgccgttc agtgtgtagt taaagacctt tttatcacca
      961 aaagagactg cccactcgcg tgcgaagctg ttcgggctac agtgctgttt aattgttccg
     1021 tcgctttcct gccactgtag tttgatgttt gttgaaccgt catcaatgaa taccaacat
//
