LOCUS       sequence-151696-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of single-cysteine variant of plasmid segregation 
            protein, ParM, as scaffold for ADP biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Kunzelmann S, Webb MR
  TITLE     A biosensor for fluorescent determination of ADP with high time 
            resolution.
  JOURNAL   J Biol Chem. 2009 Nov 27;284(48):33130-8. doi: 
            10.1074/jbc.M109.047118. Epub 2009 Sep 29.
  PUBMED    19801632
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.047118"; journalName: "J Biol Chem"; date: 
            "2009-11-27- 27"; volume: "284"; issue: "48"; pages: "33130-8"
COMMENT     Sequence Label: pHis17_ParM _1
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tatacatatg ttggtattca ttgatgacgg
       61 ttcaacaaac atcaaactac agtggcagga aagcgacgga acaattaaac agcactgtag
      121 cccgaacagc ttcgcacgcg agtgggcagt ctcttttggt gataaaaagg tctttaacta
      181 cacactgaac ggcgaacagt attcatttga tccaatcagc ccggatgctg tagtcacaac
      241 caatatcgca tggcaataca gcgacgttaa tgtcgttgca gtgcatcacg ccttactgac
      301 cagtggtctg ccggtaagcg aagtggatat tgtttgcaca cttcctctga cagagtatta
      361 cgacagaaat aaccaaccca atacggaaaa tattgagcgt aagaaagcaa acttccggaa
      421 aaaaattaca ttaaatggcg gggatacatt cacaataaaa gatgtaaaag tcatgcctga
      481 atctataccg gcaggttatg aagttctaca agaactggat gagttagatt ctttattaat
      541 tatagatctc gggggcgcca acttagatat ttctcaggta atggggaaat tatcggggat
      601 cagtaaaata tacggagact catctcttgg tgtctctctg gttacatctg cagtaaaaga
      661 tgccctttct cttgcgagaa caaaaggaag tagctatctt gctgacgata taatcattca
      721 cagaaaagat aataactatc tgaagcaacg aattaatgat gagaacaaaa tatcaatagt
      781 caccgaagca atgaatgaag cacttcgtaa acttgagcaa cgtgtattaa atacgctcaa
      841 tgaattttct ggttatactc atgttatggt tataggcggt ggcgcagaat taatagccga
      901 tgcagtaaaa aaacacacac agattcgtga tgaacg
//
