LOCUS       sequence-151705-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Menade M, Kozlov G, Trempe JF, Pande H, Shenker S, Wickremasinghe S,
            Li X, Hojjat H, Dicaire MJ, Brais B, McPherson PS, Wong MJH, Young 
            JC, Gehring K
  TITLE     Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin 
            provide insight into pathological mutations.
  JOURNAL   J Biol Chem. 2018 Aug 17;293(33):12832-12842. doi: 
            10.1074/jbc.RA118.003939. Epub 2018 Jun 26.
  PUBMED    29945973
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.003939"; journalName: "J Biol Chem"; date: 
            "2018-08-17- 17"; volume: "293"; issue: "33"; pages: "12832-12842"
COMMENT     Sequence Label: pGEX6P1-Ubl
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(313..336)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(343..996)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(363..385)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(1008..1030)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1019..1035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1043..1049,1050..1065,1066..1071))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gaaacgcgcg aggcagatcg tcagtcagtc acgatgcggc cgctcgagtc acccttttaa
       61 gccttttgat tgaaggttta caaaaagatg acaatttttg gaagtcagat ctccaatctt
      121 gatccagtca gataactcgc ggccgccgcg ccacagccgc tgctccgaca ccgggaagcc
      181 agtctccgcg aagatacgtt ccttcacatc gcgcacggtc caggacgcca gcgccgcgac
      241 ggtcctgcag cccacgcagc cggggagcac ggtcaccggg acccacctgt tctccttggt
      301 ctcggatccc aggggcccct ggaacagaac ttccagatcc gattttggag gatggtcgcc
      361 accaccaaac gtggcttgcc agccctgcaa aggccatgct atatacttgc tggatttcaa
      421 gtacttatca atttgtggga tagcttcaat acgtttttta aaacaaacta attttgggaa
      481 cgcatccagg cacattgggt ccatgtataa aacaacatca agagcgtcat acaacatgaa
      541 gtcaggatgg gttacatgat caccatttaa atatgtttta tgacataaac gatcttcgaa
      601 cattttcagc atttcaggta gcttgctaag aaaatcaact ttgagagttt caaagtcttt
      661 actatatgca attctcgaaa caccgtatct aatatccaaa accgctcctt caagcattga
      721 aatctctgca cgctcttttg gacaaccacc caacatgttg tgcttgtcag ctatataacg
      781 tatgatggcc atagactgtg ttaatttaac atcaccatca atataataag gaagattggg
      841 aaactccaaa cccaattcaa actttttgtt tcgccattta tcaccttcat cgcgctcata
      901 caaatgctct tcatattttt cttcaagata ttccaaaaga agtcgagtgg gttgcacaag
      961 gcccttaatt ttccaataac ctagtatagg ggacatgaat actgtttcct gtgtgaaatt
     1021 gttatccgct cacaattcca cacattatac gagccgatga ttaattgtca acagctcatt
     1081 t
//
