LOCUS       sequence-151718-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of double-cysteine variant of plasmid segregation 
            protein, ParM, as scaffold for ADP biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Kunzelmann S, Webb MR
  TITLE     A fluorescent, reagentless biosensor for ADP based on 
            tetramethylrhodamine-labeled ParM.
  JOURNAL   ACS Chem Biol. 2010 Apr 16;5(4):415-25. doi: 10.1021/cb9003173.
  PUBMED    20158267
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2010-04-16- 16"; volume: "5"; issue: "4"; 
            pages: "415-25"
COMMENT     Sequence Label: pHis17_ParM _2
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgttggta ttcattgatg acggttcaac
       61 aaacatcaaa ctacagtggc aggaaagcga cggaacaatt aaacagcaca ttagcccgaa
      121 cagcttcgca cgcgagtggg cagtctcttt tggtgataaa aaggtcttta actacacact
      181 gaacggcgaa cagtattcat ttgatccaat cagcccgtgt gctgtagtca caactaatat
      241 cgcatggcaa tacagcgacg ttaatgtcgt tgcagtgcat cacgccttac tgaccagtgg
      301 tctgccggta agcgaagtgg atattgtttg cacacttcct ctgacagagt attacgacag
      361 aaataaccaa cccaatacgg aaaatattga gcgtaagaaa gcaaacttcc ggaaaaaaat
      421 tacattaaat ggcggggata cattcacaat aaaagatgta aaagtcatgc ctgaatctat
      481 accggcaggt tatgaagttc tacaagaact ggatgagtta gattctttat taattataga
      541 tctcgggggc gccaacttag atatttctca ggtaatgggg aaattatcgg ggatcagtaa
      601 aatatacgga gactcatctc ttggtgtctc tctggttaca tctgcagtaa aagatgccct
      661 ttctcttgcg agaacaaaag gaagtagcta tcttgctgac tgtataatca ttcacagaaa
      721 agataataac tatctgaagc aacgaattaa tgatgagaac aaaatatcaa tagtcaccga
      781 agcaatgaat gaagcacttc gtaaacttga gcaacgtgta ttaaatacgc tcaatgaatt
      841 ttctggttat actcatgtta tggttatagg cggtggcgca gaattaatag
//
